Key Area 1.2 - Replication Of DNA

Question 1

3’ - 5’

Answer 1

Strand of nucleic acid running from a sugar to a phosphate

Question 2

DNA polymerase

Answer 2

Enzyme that adds free complementary DNA nucleotides during replication of DNA

Question 3

Heat tolerant DNA polymerase

Answer 3

Enzyme from hot-Spring bacteria used in PCR

Question 4

Lagging strand

Answer 4

DNA strand that is replicated in fragments

Question 5

Lead strand

Answer 5

DNA strand that is replicated continuously

Question 6

Ligase

Answer 6

Enzyme that joins DNA fragments to make the lagging strand

Question 7

Negative control

Answer 7

Part of experimental design in which a negative result is expected; in PCR, a tube with no DNA to be amplified

Question 8

Polymerase chain reaction

Answer 8

Method of amplifying sequences of DNA in vitro

Question 9

Positive control

Answer 9

Part of experimental design in which a positive result is expected; in PCR, a tube with DNA which is already known to be amplified by the method being used

Question 10

Primer

Answer 10

Short complementary strand of DNA

Question 11

Replication

Answer 11

Formation of identical copies of DNA molecules

Question 12

Template strand

Answer 12

DNA strand on which a complementary copy is made