JK L2 eukaryote gene expression

Question 1

how do eukaryotes regulate gene expression…?

Answer 1

direct- interaction with transcriptional machinery

indirect- changing chromatin structure, opening up diff areas of the chromosome allowing transcription factors to get into the promoter

Question 2

what does the phosphorylation of TFIIH lead to…?

Answer 2

the start of RNA synthesis

Question 3

TATA binding protein binds….where?

Answer 3

to the core promoter to enable RNA pol II and other transcription factors to bind

Question 4

info on TATA box

Answer 4

TATA box indirectly positions RNA pol II to begin transcription which is positioned around 30bp downstream

Question 5

TF’s which will help determine the level of transcription from the preinitiation complex

Answer 5

Ø TF that bind close to promoter
Ø TF that bind far away from promoter (called enhancers)
Ø Some which will act negatively and are far away called silencers
Ø Close binders will be specific for this promoter

Far away binders might be controlling more than one promoter