Jan 16 Flashcards
Covalent mod of proteins:
1. Alters protein ____ in the cell.
2. Alters ________
3. Controls ________
4. Provides a specific ________ for other proteins or molecules
- location
- activity
- proteolytic degradation
- interaction domain
Does protein kinase need Mg on ATP to use it to phosphorylate protein?
Yes it needs Mg-ATP
There is glycogen phosphorylase b and a, which one is active and inactive and what conditions?
B is active when high concentrations of 5-AMP (low energy) and a active when high energy state (low 5’AMP). This is due to if protein is PO4’d or not for if in b or a form
What protein domains bind to the following covalent mods: PO4’d, methylation, acetylation, ubiquitylation
PO4’d = SH2 domain
Methylated lysine = Chromodomain
Acetylated lysine = bromodomain
Ubiquitinylated = ubiquitin interaction module
How many AA’s is a protein motif, domain/modules
3-10 AA’s for motif, domains/modules are 35-150 AA’s
With respect to domains and modules, what does the human genome tell us about protein evolution (2)?
- Few new inventions
- Domains and modules are used again and again (many SH2 domains)
How does Rous sarcoma virus cause cancer?
It has SRC gene obtained from host genome but it is mutated, so causes issues in host
What things to phsophotyrosine bind to (2)?
PTB (phsophotyrosine binding) domains, and SH2 domains
What things do PO4’d serines/threonines bind to (3)?
FHA for pThr, MH2 for both, BRCT for pSer
What are some examples of modulator proteins?
Kinases, phosphatases, adaptors, scaffolds, transcription proteins etc. All of these have mutliple interaction domains, so the protein can mediate multiple interactions between proteins and phospholipids, carbohydrates, and nucleic acids
What domains bind to phosphoinoditides?
PH domains
