April 1 Flashcards
mTORC1 moves to what compartment in response to what biomolecule(s) due to what protein. If this protein is always GTP bound, then will mTORC1 stay or not be in compartment all of time?
Will move to lysosome when have amino acids. RagB is GTP loaded when AAs are present, which enables mTORC1 to attach to lysosome surface. If RAG A/B is always GTP loaded, then mTOR will stay at lysosome surface as it is insensitive to amino acid starvation
The Ragulator complex is the ____ for RAGS?
GEF, so promote GTP instead of GDP bound
RAG A/B is bound to Rag C/D. Which is GDP and GTP loaded before/after adding AA’s? What is the consequence/
If AA starvation, then RagA/B is GDP loaded, and Rag C/D is GTP loaded. When add AA’s, then AB now GTP, and CD is GDP. This enables mTORC1 to bind to them and be activated by Rheb on the lysosome surface
Ragulator and Rheb are attached by lysosome by _______
Lipid modifications, permanently attached to MB
What is the RAPTOR Claw? Where is it located?
It is a 22 AA loo, it is between Rag A/B, and Rag C/D.
What is the GAP for Rags? What does its antagonistic partner, what does it do?
It is GATOR1 complex, transmit cytosolic AA signal to Rags.
GATOR2 inhibits GATOR1 when leucine is present, therefore Rag A/B stays GTP loaded, so mTORC1 still at lysosome
What does SLC protein stand for?
It is Solute carrier protein
What does SLC38A9 sense?
This solute carrier senses Arg levels in lysosome.
What senses leucine starvation, and does what after?
Sestrin2 in cytosol senses leucine. When leucine starved, it binds and inhibits GATOR2. GATOR2 usually inhibits GATOR1 (GAP for Rag A/B), but with G2 inhibited then the GAP can do its thing and prevent TORC1 from getting recruited to lysosome.
What senses S-adenosylmethionine?
SAMTOR
What senses Arg in the cytosol?
CASTOR1
