Isolation Of Streptococcus Salvarius Flashcards

1
Q

What is the process called that converts nitrogen gas (N2) into ammonia?

A

Nitrogen fixation

This process is crucial for creating organic nitrogen needed for life.

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2
Q

Which group of organisms carries out nitrogen fixation?

A

Prokaryotes

This includes many soil bacteria such as Azotobacter, Clostridium, and Klebsiella.

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3
Q

What role do soil microorganisms play in the decomposition of organic matter?

A

They degrade organic matter to produce nutrient-rich topsoil

This process is essential for recycling nutrients in ecosystems.

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4
Q

What is a beneficial practice encouraged for managing yard waste?

A

Composting

A good compost pile requires a balance of leafy and grassy wastes to support microorganisms.

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5
Q

Name an antibiotic produced by the fungus Penicillium.

A

Penicillin

This antibiotic was the first widely used and has saved countless lives.

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6
Q

Which genus of bacteria produces streptomycin?

A

Streptomyces

This genus also synthesizes geosmins, contributing to the earthy smell of soil.

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7
Q

What is the dormant stage formed by Bacillus and Clostridium in response to adverse conditions?

A

Endospore

Endospores are highly resistant and can survive extreme conditions.

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8
Q

How long does it take for endospore formation to occur?

A

About eight hours

Mature endospores can remain viable for at least 50 years, potentially thousands.

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9
Q

What distinguishes Clostridium species from Bacillus species?

A

Oxygen requirements

Clostridium are obligate anaerobes, while Bacillus can be obligate aerobes or facultative anaerobes.

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10
Q

True or False: Most soil microbes are harmful and can cause diseases.

A

False

While most soil microbes are beneficial, some can cause diseases like tetanus and botulism.

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11
Q

What are the causative agents of tetanus and botulism?

A

Clostridium tetani and Clostridium botulinum

These bacteria can produce potent toxins under anaerobic conditions.

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12
Q

What technique will be used to estimate the number and diversity of aerobic microorganisms in the soil?

A

Spread plates

This method avoids the high temperatures that can kill environmental organisms.

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13
Q

How are Bacillus endospores counted in the soil sample?

A

By heating the sample and then plating it

Heat treatment kills vegetative cells but not endospores, allowing for accurate counting.

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14
Q

Fill in the blank: The characteristic ‘earthy’ odor of soil is produced by _______.

A

Streptomyces

These bacteria produce geosmins, which contribute to the soil’s smell.

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