Aseptic Technique

Question 1

Where is the hottest region of the burner?

Answer 1

The tip.

Question 2

What do you do with the cap of a bacterial you are trying to sample?

Answer 2

KEEP IT IN YOUR HAND not on the desk.

Question 3

When should you put a Pipette (out of sterile packaging) on the counter?

Answer 3

Never, either in your hand or in biohazard container.

Question 4

What’s the purpose of heat fixing?

Answer 4

To make organisms stick to the glass slide.