Iron Studies And Blood Bank Flashcards
What is used to transport iron around blood
Transferrin
Iron outside of blood is stored in what type of cells
Ferritin
Serum iron
Measures amount of iron bound to transferrin in serum
W/ iron deficiency = LOW
TIBC
Measures binding capacity of transferrin
Increased in patients with iron deficient anemia (more desperate – less iron you actually have, more “room” to bind)
Transferrin saturation
Index of iron saturation
Less iron you have more capacity you have to bind – SO low saturation in Fe deficient anemia
Ferritin lab
Iron storage protein
Studied as a reflection of iron storage in the body
LOW in Fe def. anemia
Direct way to study iron stores in body
Direct liver or bone marrow bx
Not done bc invasive
Use Ferritin lab instead
Hemosidrin
Storage of iron in the liver and bone marrow
Studied in direct biopsy and stained with Prussia Blue
Most basic serologic test
Agglutination
Particulate coated with Ag
Ab cross link the antigen, clumping occurs
Type A blood
A antigen on blood - doesn’t react
Anti B antibodies (will react if given B blood)
Can receive A, O
Type B
B Ag on RBCs
Anti-A Abs (will react)
Can receive: B, O
Type AB
Both A and B antigens on surface
No antibodies
UNIVERSAL RECEIVER
Type O
No A or B antigens on surface
Has A and B antibodies in serum
UNIVERSAL DONOR
D antigen
Rh factor
D+ = present (Rh +)
If D-, exposure to D will have terrible results
Other Ags in blood
Rh Duff MNS Kell Kidd P
Detected by COOMBS or IAT
Type and screen
ABO type and screen for common abs
Just screening patients blood to see if it has Abs in it
Used if might need transfusion
type and cross match
Mix patient blood and donor serum and determine if they will be compatible
Confirms AB, Rh typing quickly
Detects other Abs and those that don’t agglutinate easily
HLA testing
Class I and II
Regulate immune response
Play a role in blood donation, organ donation, and transplantation
Hemolytic reactions
Specific destruction of transfused RBC by recipient Abs
Typically a transfusion reaction
COOMBS
Detects Abs in RBCs
Detect if you made antibodies to transfused RBC and is coated on RBC
IAT COOMBS
See if patient has developed new antibodies
Same test but used at opposite times?
