Anemia Flashcards
Oxygen carrying unit
Heme
Contains iron
Also assists in CO2 transport (can pick up CO, NO)
Laboratory definition of anemia
Hg < 12 g/dL (women), <13 (men)
Hct < 36%, <40%
Can be asymptomatic or symptomatic
General s/s of anemia
Pallor (conjunctiva, skin, membranes)
Tachycardia
Orthrostasis
Weakness, fatigue
How is anemia diagnosed? (Test and associated)
CBC
Using parameters
RBC, MCV, RDW, MCHC
MCV
Tells us the AVERAGE size of the cells
Microcytic (<85)
Normocytic (85-100)
Macrocytic (100+)
What indicies must we use in addition to the MCV to diagnose anima ?
RDW
Tells us the distribution of the cells (11-16% is good)
Narrow RDW = less size difference between the cells
Retic count
Dependent upon degree of anemia
Used in anemia to see if the bone marrow is working
Would expect low if bone marrow not working effectively
Corrected retic count
Bc anemia will give us a typically higher retic count than average we must correct it
Corrected retic > 2%
Normal proliferation
Could be hemolytic or due to acute blood loss
Corrected retic < 2%
Hypoproliferation in bone marrow
Tests comprising iron studies (5)
- Iron (iron levels in serum)
- Transferrin (amount of transferrin in blood)
- TIBC (available transferrin in blood)
- % sat (amount of transferrin with iron)
- Ferritin – impacted by inflammation or infection
Additional tests to evaluate anemia (after iron studies)
B -12 and folate levels
LFTs (specifically LDH)
Haptaglobin
TSH
SPEP/UPEP
DAT
BM bx
Hg.Genetic testing
Mild anemia workup
- Evaluate for heavy blood loss
- Order initial work up (iron studies, b-12 and folate)
- Treat or move towards urgent eval
Severe anemia evaluation
- Evaluate for blood loss (GI bleed – not menstrual at this point)
- Order initial work up + LDH, haptaglobin, retic, DAT
Target cells
Peripheral blood smear
Caused by membrane defect
Thalassemia or liver dz
Peripheral blood smear
Schistocytes
Helmet fragments
Seen in INTRAVASCULAR hemolytic processes (DIC)
Peripheral blood smear
Basophilic stippling
Blue stipples appearance
Seen in lead toxicity (microcytic anemia)
Peripheral blood smear
Rouleaux formation
RBC staked like coins
Due to high paraprotein in blood
Indicates multiple myeloma
Types of microcytic anemias (5)
- Iron deficiency anemia
- Lead poisoning
- Anemia of chronic disease
- Sideroblastic anemia
- Thalassemia
Where is iron absorbed from
Proximal duodenum and moved thru blood by transferrin to marrow
When RBCs are broken down in liver, iron is recycled and carried to marrow
Small amount of iron is lost daily but most of it is retained
MC cause of microcytic anemia
Iron deficiency
Typically hypochromia and can cause thrombocytosis
Epidemiology of IDA
Women of childbearing age (heavy menses)
Vegetarians, infants, pregnancy women, celiac or IBDs
Conditions/symptoms associated with IDA
PICA
RLS/leg cramps
Cold intolerance
Esophageal webs
Chelitits
Koilonychia
Etiology of IDA
Iron loss (menstruation, GI bleed, chronic low grade hemolysis)
Insufficient absorption (poor intake, malabsorption by gut)
IDA
CBC results
Hypochromia
microcytic anemia
+/- thrombocytosis
Peripheral smear
IDA
No structural abnormalities
Uniform size – just small
Iron studies
IDA
Iron: LOW
** Transferrin: HIGH
TIBC: high
% saturation: LOW
Ferritin: LOW
IDA workup (4 steps)
- Women with heavy periods – begin Fe replacement, check
- M/f w/o menses - suspect GI bleed so endoscopy, colonoscopy
- No finding of blood loss yet, will do capsule scope to look at SI
- Nothing yet, workup for malabsorption (celiac)
IDA treatment
Oral Ferrous sulfate (325 mg) given up to 3x/day
Should be taken with vitamin C on empty stomach (to maximize absorption)
Other formulations are available but not great (parentarel venofer if nec. - low anaphylaxis)
How long on IDA treatment before results are seen?
Retic count should increase in 5-10 days, HgB should increase 1-2 gm/2 weeks
Typ. Takes 3-4 months to get to normal
Prevention is done by taking supplemental iron (infants, vegetarians, pregnant)
Caution in treating IDA + remedy
Iron overload
Can happen in transfusion (200mg/unit PRBC)
Can’t get rid of excess iron so will store in improper organs
TReAMENT: chelation or phlebotomy
Disorder caused by genetic defect causing decrease in alpha or beta chain production
Thalassemia
African, Asian, Mediterranean
Alpha thalassemia
Impaired alpha chain production – too many beta chains
May allow malaria prevention
Four subtypes
What does the HgB look like in alpha thalassemia
The Beta chains can form stable tetramers so they will get out into the serum (little effect on erythropoiesis)
BUT beta chains have a high O2 affinity so it can hold, but not release oxygen
Types of alpha thalassemia
- Alpha thalassemia minima
- Alpha thalassemia minor/trait
- Alpha thalassemia/HgH
- Fetal Hydrops
Alpha thalassemia minima
Deletion of 1 alpha gene
Silent carrier
No abnormalities in smear or CBC
Alpha thalassemia Minor
2 genes deleted
Minimal anemia with reduced MCV (minor microcytic anemia)
Alpha thalassemia/ HgH
Significant production of beta tetramers
Microcytic anemia and intravascular hemolysis
Target cells and Heinz bodies on smear
Fetal Hydrops
4 alpha genes are missing
Incompatible with life
HgH disease tetramers (+ symptoms)
B chain tetramers
Soluble but not stable when under stress
They precipitate out but are hemolyized in the vessels
Significant anemia, jaundice with papal leaders splenomegdaly
Significant reticulocytosis
Beta thalassemia
Suppression of beta chain production resulting in over production of alpha chains
Alpha chains are not able to form tetramers so they are destroyed in the marrow
Types of beta thalassemia
- Beta thalassemia minor
2. Beta thalassemia major
Beta thalassemia minor
CBC
Asymptomatic but significant microcytosis
Normalish Hct, Narrow RDW
High RBC counts