Investigating Membrane Permeability

Question 1

How do you investigate membrane permeability?

Answer 1

use a colourful tissue e.g. beetroot

wash thoroughly

range of at least 5 either water baths at diff temps or solvent concentrations e.g. alcohol

control all other variables e.g. length of time / size of betroot

accurately measure volumes e.g. volumetric pipette

colour is determined using a colorimeter set to absorbance

use a distilled water blank

repeat at least 3 times

take mean balue (calc mean)