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Micro Test I > Introduction to Medical Virology > Flashcards

Introduction to Medical Virology Flashcards

1
Q

Properties of viruses

A

acellular infectious agent
a nucleic acid surrounded by a protein coat
obligate parasite

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2
Q

Naked capsids

A

are very environmentally stable
released from cell by lysis
Are very resistant

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3
Q

Envelope viruses

A

way easier to kill

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4
Q

Ways to disrupt envelope viruses

A

acid
detergents
drying
heat

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5
Q

ssDNA

A

parvovirdae

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6
Q

dsDNA

A 
Herpesviridae
Hepadnaviridae
Adenocviridae
Papovavairdae
Parvoviridae, 
Poxviridae
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7
Q

dsRNA

A

reoviridae

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8
Q

-ssRNA

A

a. Orthomyxoviridae
b. Paramyxoviridae
c. Rhabdoviridae
d. Bunyaciridae
e. Arenaviridae
f. Filoviridae

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9
Q

+ssRNA

A

a. Togaviridae
b. Flaviviridae
c. Coronaviridae
d. Retroviridae
e. Picornaviridae
f. Caliciviridae
g. Hepeviridae

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10
Q

components of virion

A

a. Viral attachment proteins
b. Envelope
c. Matrix/tegument
d. Capsid
e. genome

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11
Q

typical steps in viral replication

A

1) Individual viral proteins form into capsid subunits
2) Subunits combine to form complete capsid
3) Viral genome and other essential virion components are selectively packaged into capsids
4) Virus exits cell

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12
Q

Recombination

A

fast changes

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13
Q

reassortment

A

dramatic changes in their genetic makeup

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14
Q

What does virus culture/plaque assay detect

A

infectious virus

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15
Q

What are the advantages of virus culture/plaque assay

A

positivity shows active viral infection

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16
Q

What are the limitations of virus culture/plaque assay

A

restricted to viruses that replicate in tissue culture and produce cytopathic effect

17
Q

What does electron microscopy detect

A

virion particles

18
Q

Advantages of electron microscopy

A

helpful in identification of emerging viruses

19
Q

Limitations of electron microscopy

A

expensive and difficult to do

20
Q

what does ELISA detect

A

viral proteins and glycoproteins

21
Q

What are advantages of ELISA

A

sensitive and quick

22
Q

What are the limitations of ELISA

A

requires specific antibody

23
Q

What does PCR detect

A

DNA genomes

24
Q

What are the advantages of PCR

A

highly sensitive

25
Q

What are the limitations of PCR

A

DNA sequence information must be available

26
Q

What does RT-PCR detect

A

RNA genomes

27
Q

What are advantages of RT-PCR

A

highly sensitive

28
Q

What are limitations of RT-PCR

A

RNA sequence information must be available

29
Q

What does Serology (western blot) detect?

A

anti-viral antibodies

30
Q

What are advantages of serology (western blot)

A

sensitive and quick

31
Q

What are limitations of serology (western blot)

A

takes time to figure out what you are looking for

Micro Test I (10 decks)

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