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Introduction to CC Flashcards

1
Q

a solution with known concentration, used to as reference to determine the concentration of unknown solution. Prepared by dissolving accurately weigh pure substance in a definite volume of solvent.

A

Standard solution-

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2
Q

highly purified chemical (99%) with known concentration and purity that is commercially available. It used as a reference standard.

A

Primary standard (reagent grade)

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3
Q

very high purity, equal or exceeds the specification of American chemical society (ACS). For laboratory use, bottles have AR or ACS initial.

A

Analytical reagent
(reagent grade)

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3
Q

prepared from primary standard and specifically used for a specific analysis and calibration of instrument.

A

Secondary standard-

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4
Q

Nano grade. For high-end laboratory exams such trace element determination, HPLC, immunoassays and molecular technique.

A

Ultra pure

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4
Q

Limits of impurities are not specified. Not recommended for lab examination but may be use in general chemistry and less critical analysis.

A

Chemically pure (laboratory grade)

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5
Q

Not necessarily of sufficient purity use as analytic grades. Used for drug manufacture.

A

National formulary

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6
Q

Not necessarily of sufficient purity use as analytic grades. For industrial use

A

Commercial or technical grade

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6
Q

According to CAP
For examination require maximum of highest purity like special chemistry assays, microbiology and cell or tissue culture.

A

Type I
(reagent grade water)

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7
Q

According to CAP
Preparation of calibrators and standard solution.

A

Type I
(reagent grade water)

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8
Q

According to CAP
For routine examination in chemistry, microbiology, immunology and hematology

A

Type II
(pure water)

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9
Q

According to CAP
Produced by reverse osmosis or distillation.
For qualitative measurement such as parasitology, histology and clinical microscopy.
For washing of glasswares and autoclave.

A

Type III
(instrument-feed water)

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10
Q

According to CSLI
Purification is by combination of distillation, deionization, reverse osmosis and filtration.

A

Clinical laboratory reagent grade (CLRW)

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11
Q

According to CSLI
For routine testing

A

Clinical laboratory reagent grade (CLRW)

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12
Q

According to CSLI
Used in automated machines for rinsing and dilutions.
For water bath

A

Instrument-feed water

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13
Q

According to CSLI
For examination such as DNA/RNA analysis and trace metal metals.

A

Special reagent water (SRW)

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14
Q

Glassware
High resistance to thermal shock and chemical attack. Used for beakers, flask, pipets and test tubes.
Can be heated and autoclave.
Most commonly used.

A

Borosilicate glass
Example: Pyrex and kimax

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15
Q

Example of Borosilicate glass

A

Pyrex and kimax

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16
Q

Stronger than borosilicate. Can resist clouding and scratching.

A

Aluminosilicate glass

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17
Q

Plastic, Widely used for making test tubes, disposable transfer pipet and test tube racks

A

Polyethylene or Polypropylene

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17
Q

Acid, thermal and heat shock resistant used for disposable materials such as pipet tips it is chemically resistant and can be autoclaved.

A

Vycor

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18
Q

Plastic, used in tubes for centrifugation, graduated cylinders and flask.

A

Polycarbonate

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19
Q

Soda-lime glass with oxides of sodium, silicon and calcium. Used for some disposable glassware such as pipet tips.

A

Flint glass

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19
Q

Amber or red color. Used to reduce light exposure such as bilirubin standard.

A

Low actinic

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20
used for transferring liquid, made up of glass or plastics can be reusable or disposable.
Pipettes
21
contains a particular volume but does not dispense the exact volume due to tendency of fluid to cling to glass surfaces. Also referred as rinse-out pipet
To contain pipette
22
dispense the exact volume indicated.
To deliver (TD) pipette
23
pipette for hemoglobin determination
sahli pipette
24
dispense one volume without further subdivision (self draining )
Transfer pipet
25
Types of transfer pipette
Volumetric Oswald-Folin Pasteur Automatic
26
bulb-like enlargement pipette. Self-draining used to transfer aqueous solution.
Volumetric pipets-
27
bulb closer to the delivery tip. use to transfer biological fluids with greater viscosity than water.
Oswald-Folin pipet-
28
used to transfer biologic fluid or solution without specified volume. No graduated mark.
Pasteur pipettes-
28
with graduation mark at the tip and usually blow out.
Serologic pipet
29
most commonly used in Clinical Chemistry
Automatic pipette-
29
deliver the amount of liquid contained between two calibration marks.
Measuring or Graduated pipet-
29
self draining pipet without graduation marks at the tip. Self-draining pipette.
Mohr pipet-
30
Types of Measuring or Graduated pipette
Mohr Serologic Bacteriologic, Ball, Kolmer or Khan Micropipette
31
Has 2 close rings mark at the top of the pipette. Similar with TD pipette.
Blow out
32
No markings at the top. Allows the content to dispense by gravity.
Self draining
33
first micropipette and probably the most accurate ever developed
Lang-Levy Pipets(TC)
34
most commonly used micropipette
Piston-operated Pipets (TD)
35
with disposable tips placed on the end and a piston pulls up a calibrated volume
Piston-operated Pipets (TD)
36
example of Piston-operated Pipets (TD)
Eppendorf
37
micropipette uses reusable tips
Positive displacement Pipets (TD)
38
Types of micropipettes
Lang-Levy Pipets(TC) Piston-operated Pipets (TD) Positive displacement Pipets (TD)
39
depends on piston to create a suction draw.
Air Displacement
40
piston is move within the barrel or tip to create suction.
Positive-displacement
41
obtain a liquid from a reservoir and dispense it repeatedly.
Dispenser pipette
41
What method of pipette calibration based on weight of sample - TD makes use of water(Type I); TC makes use of mercury
Gravimetric Method
41
What method of pipette calibration for micropipets - use colored compound of known absortivity like 4-nitrophenol
Spectrophotometric Method
42
What method of pipette calibration for micropipets - use gamma-emitting radioisotopes like Iodine-125
Use of Radioisotopes
43
Calibration of pipette
Twice yearly
44
Used in the laboratory for monitoring the temp of refrigerator, freezers, water bath, heating blocks and incubator.
Thermometer
45
Types of thermometer
Total immersion Partial immersion Surface immersion
46
Thermometer should be verified for accuracy ?
6 or 12 months interval
47
Thermometer used for refrigerators and freezer
Total immersion
48
Thermometer used for water baths
Partial immersion
49
Thermometer used for incubator and heating oven.
Surface immersion
50
Device to accelerate gravitational separation of substances with different densities. Separates solid from liquid suspension.
Clinical centrifuge
51
Use to separate liquid phase of different densities like the separation of lipid compounds from plasma or serum.
Clinical centrifuge
52
tubes assume a horizontal plane when rotor is in motion and vertical when at rest. Capable of speed up to 3000 RPM.
Horizontal head or Swinging bucket
53
tubes are held at fixed position at angles from 25-40 degrees to the vertical axis of rotation.
Angle head rotor type
54
more heat generated so with refrigerated chamber -Typically use in blood banking for the separation of blood components. - Used to separate lipoproteins in Clinical Chemistry.
Ultracentrifugation
55
Calibration of Centrifuge
quarterly
56
designed to reduce potential illness or injuries brought about by toxic chemicals.
Material safety data sheet (MSDS)
57
Blue quadrant
Health
58
Red Quadrant
Flammability
59
Yellow Quadrannt
Reactivity/Stability
60
White quadrant
Special/Specific information
61
It is used for alerting personnel of the degree of hazard of the chemical and helpful in drawing attention to storage needs and the necessary emergency equipment needed.
NFPA(national fire protection association) hazard diamond.
62
Examples of Acids
Formic acid, nitric acid, sulfuric acid, hydrochloric acid, glacial acetic acid
63
Examples of Bases
NH4OH, KOH, NaOH
64
Examples of Flammables
Acetone, alcohol, xylene
65
Examples of Oxidizers
Hydrogen peroxide, KCl, acetic acid
66
Examples of water-reactive chemicals
Na, K
67
Class A materials and type of extinguiser
cloth, paper, wood liquids Pressurized water Dry chemicals
68
Class B materials and type of extinguiser
hydrocarbons, flammable liquids Dry chemicals, Carbon dioxide foam or halon
69
Class D materials and type of extinguiser
combustible metal Leave to professional fire fighter
69
Class C materials and type of extinguiser
electrical equipment Dry chemicals Carbon dioxide or Halon
70
Class K materials and type of extinguiser
Grease, oil and fats Liquid designed to prevent splashing and cool the fire
71
RACE
R- rescue the person in danger A- activate the fire alarm C- contain the fire E- extinguish the fire
72
PASS
P- pull the pin A- aim at the base of fire S- squeeze the handle S- sweeping (side by side motion)

CC 1 (6 decks)

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