CARBOHYDRATES II Flashcards
can be used as a sample.
Whole blood, serum , plasma, CSF, serous fluid or urine
preservative in evacuated tubes that prevents glycolysis and stable up to 24 hours at room temperature
sodium fluoride
preservative in evacuated tubes that prevents clotting
potassium oxalate
evacuated tube that contains sodium fluoride and potassium oxalate.
Evacuated gray top tube
most commonly used specimen
Serum
Fasting required is at least?
8 hour not longer than 16 hours
Serum at room temperature, glucose concentration is stable up to ____ hours and up to ____ hours at _____
8
72
4C
specimen that should be process immediately to avoid bacterial contamination.
CSF glucose
Urine glucose in 24-hour specimen can be preserved by adding?
55mL glacial acetic acid
Higher glucose level in capillary blood, ____ higher than venous blood
2-3%
Blood glucose determination
Chemical Method
Enzymatic method
Dextrostix (ONLY ONE)
Chemical method
1. oxidation reduction method
2. condensation method
Enzymatic method
1. glucose oxidase
2. hexokinase
3. glucose dehydrogenase
In alkaline pH Cupric ions is reduced to cuprous ion in the presence of reducing sugar.
Copper reduction method
Most carbohydrates are reducing sugar except
sucrose
Folin wu method (benedict’s test)
Reagent and Result
Reagent: phosphomolybdate
Result: molybdenum blue
Nelson-Somogyi
Reagent and Result
Reagent: arsenomolybdate
Result: molybdenum blue
Neocuproine method
Reagent and Result
Reagent: neocuproine
Result: yellow-orange red complex
( Hagedorn Jensen method)
also known as
Ferric cyanide method
Ferric cyanide method is also known a s
Hagedorn Jensen method
reduce to ferrocyanide in the presence of reducing sugars
Ferric cyanide
From colored reagent (yellow) to colorless and measured at 400 nm.
Ferric cyanide
Reagent of Ferric cyanide method
Regent: hot alkaline solution of ferric cyanide.
Chemical method Used in automated machines.
Ferric cyanide method
Uses hot acidic medium to produce “enol” form of glucose which is the active reducing form of glucose to convert D-glucose to L-glucose.
Ortho -tuloidine method (dubowski method)
(dubowski method) is also called
ortho- tuloidine method
What method?
Glucose + Glacial acetic + heat at 100C = green color measured at 620-630 nm
Condensation method
Ortho -tuloidine method (dubowski method)
The most common method (Enzymatic method), measures D-glucose.
Glucose oxidase method (GOD)
Hexokinase method (HK)
Glucose dehydrogenase method (GDH)