Intro to histology

Question 1

ience concerned with the morphology of the human body

Answer 1

Human Anatomy

Question 2

Histology

Answer 2

Histology

Question 3

study of tissues regarded as the microscopic study of the morphology of the human body (as seen under the microscope).

Answer 3

Histology

Question 4

macroscopic study of the morphology of the human body (as seen by the naked eye).

Answer 4

Gross Anatomy

Question 5

study of the microscopic structure of organs, tissues, and cells

Answer 5

CYTOLOGY

Question 6

most commonly used tool in basic histology

Answer 6

Lm microscope

Question 7

often a useful accompaniment (adjunct) because knowledge of the ultra structure of cells and tissues is oftentimes necessary for a more thorough understanding of their architecture

Answer 7

The electron microscope (EM)

Question 8

*Most common electron microscope

Answer 8

Transmission Electron Microscope

Question 9

agnifying the image that was taken by a microscope beyond the microscope’s resolving power. The image becomes bigger, but no additional details will be revealed.

Answer 9

Empty Magnification

Question 10

he shortest distance at which two points can be seen as separate.

Answer 10

RESOLVING POWER OF LIGHT AND ELECTRON MICROSCOPES

The resolving power

Question 11

resolving power of LM

Answer 11

0.2

Question 12

4 movable lens of LM

Answer 12

scanning 4x
lpo 10x
hpo 40x
OIO 100x

Question 13

has 2 ocular lenses each having the same magnification power.

Answer 13

Binocular Microscope

Question 14

parts of the microscope

Answer 14

• ocular lens
• body tube
• revolving nosepiece
• arm
• objectives
• stage clips
• stage
• diaphragm
• coarse adjustment
• fine adjustment
• base
• light

Question 15

what is the specimen seen in the microscope

Answer 15

thinly sliced where light passages thru

2d presentations

Question 16

PREPARATION OF TISSUE SPECIMEN FOR LIGHT MICROSCOPY

Answer 16

• the specimen to become firm enough in order to allow very thin
• slice less than 10cm
• ## slices stained

Question 17

Most common staining procedure

Answer 17

hematoxylin and eosin (H&E)

Question 18

Binds with acidic structures
Blue stains = basophilic
Ex: ribosomes (RNA)
Nuclei (RNA & DNA)

Answer 18

Hematoxylin

Question 19

Binds with basic structures
Red stains = acidophilic/ eosinophilic
Ex: mitochondria
       Cytoplasmic granules (present in intestinal Paneth cells,   
       acedophils of adenohypophysis; eosinophils in blood)

Answer 19

Eosin- Red Acidic

Question 20

magenta stain ( glycogen & carbohydrate-rich molecules)

Answer 20

Periodic acid-Schiff stain

Question 21

red stain ( glycogen)

Answer 21

Best’s carmine stain

Question 22

black (reticular fibers)

Answer 22

Silver stain

Question 23

electromagnets which focuses beam of electrons on the sample tissue/specimen.

Answer 23

ELECTRON MICROSCOPE

Wavelength of electrons = 10,000x shorter in comparison to visible light
Modern electron microscopes can magnify as much as 150,000x (visualize macromolecules)

Question 24

2 types of electron microscope

Answer 24

• Transmission Electron Microscope (EM; TEM)

- Scanning Electron Microscope (SEM)

Question 25

Allows for high magnification of surface of solid specimen
Shows 3D view
electron beam scans specimen surface which is coated with a heavy metal (Gold or Palladium) prior to the scan
Electron detectors capture ejected/reflected electrons from the heavy metal coating.

Answer 25

Scanning Electron Microscope (SEM)

Question 26

Specimen/sections observed = very thin slices of cell, tissue or organ
Allows passage of electrons

e electron micrographs

Answer 26

Transmission Electron Microscope (EM; TEM)

Question 27

first captured by an electron sensitive film (-)

This film produces a black and white image (+) called the electron micrograph which is then available for printing

Answer 27

e electron micrographs