Intro to histology Flashcards
ience concerned with the morphology of the human body
Human Anatomy
Histology
Histology
study of tissues regarded as the microscopic study of the morphology of the human body (as seen under the microscope).
Histology
macroscopic study of the morphology of the human body (as seen by the naked eye).
Gross Anatomy
study of the microscopic structure of organs, tissues, and cells
CYTOLOGY
most commonly used tool in basic histology
Lm microscope
often a useful accompaniment (adjunct) because knowledge of the ultra structure of cells and tissues is oftentimes necessary for a more thorough understanding of their architecture
The electron microscope (EM)
*Most common electron microscope
Transmission Electron Microscope
agnifying the image that was taken by a microscope beyond the microscope’s resolving power. The image becomes bigger, but no additional details will be revealed.
Empty Magnification
he shortest distance at which two points can be seen as separate.
RESOLVING POWER OF LIGHT AND ELECTRON MICROSCOPES
The resolving power
resolving power of LM
0.2
4 movable lens of LM
scanning 4x
lpo 10x
hpo 40x
OIO 100x
has 2 ocular lenses each having the same magnification power.
Binocular Microscope
parts of the microscope
- ocular lens
- body tube
- revolving nosepiece
- arm
- objectives
- stage clips
- stage
- diaphragm
- coarse adjustment
- fine adjustment
- base
- light
what is the specimen seen in the microscope
thinly sliced where light passages thru
2d presentations
PREPARATION OF TISSUE SPECIMEN FOR LIGHT MICROSCOPY
- the specimen to become firm enough in order to allow very thin
- slice less than 10cm
- ## slices stained
Most common staining procedure
hematoxylin and eosin (H&E)
Binds with acidic structures
Blue stains = basophilic
Ex: ribosomes (RNA)
Nuclei (RNA & DNA)
Hematoxylin
Binds with basic structures
Red stains = acidophilic/ eosinophilic
Ex: mitochondria
Cytoplasmic granules (present in intestinal Paneth cells,
acedophils of adenohypophysis; eosinophils in blood)Eosin- Red Acidic
magenta stain ( glycogen & carbohydrate-rich molecules)
Periodic acid-Schiff stain
red stain ( glycogen)
Best’s carmine stain
black (reticular fibers)
Silver stain
electromagnets which focuses beam of electrons on the sample tissue/specimen.
ELECTRON MICROSCOPE
Wavelength of electrons = 10,000x shorter in comparison to visible light
Modern electron microscopes can magnify as much as 150,000x (visualize macromolecules)
2 types of electron microscope
- Transmission Electron Microscope (EM; TEM)
- Scanning Electron Microscope (SEM)
Allows for high magnification of surface of solid specimen
Shows 3D view
electron beam scans specimen surface which is coated with a heavy metal (Gold or Palladium) prior to the scan
Electron detectors capture ejected/reflected electrons from the heavy metal coating.
Scanning Electron Microscope (SEM)
Specimen/sections observed = very thin slices of cell, tissue or organ
Allows passage of electrons
e electron micrographs
Transmission Electron Microscope (EM; TEM)
first captured by an electron sensitive film (-)
This film produces a black and white image (+) called the electron micrograph which is then available for printing
e electron micrographs
