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BMS 2 > Intro to cell II > Flashcards

Intro to cell II Flashcards

1
Q

Lipid Bilayer PM hydrophobicity

A

Amphilic=Amphipathic
Hydrohobic lipids in inner
Hydrophilic groups face external

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2
Q

Integral membrane proteins

A

Traverse entire width of cell membrane

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3
Q

Peripheral membrane proteins

A

Attached to extracellular regions of PM noncovalently

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4
Q

What happens to proteins when separate lipid bilayer

A

Integral proteins associate with one leaflet or the other

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5
Q

Faces of PM

A

E and P faces

P face has many more interactions with proteins-actin

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6
Q

RBC membrane proteins

A

Stabilized by spectrin and other cytoskeletal proteins (actin)-why so many proteins are on P face

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7
Q

Hereditary spherocytosis

A

Spherical/less deformable/more susceptible to destruction by phagocytic cells-RBCs-spectrin mutations

-essentially use SPECTRINS to keep membrane shape and ensure no problems with extracellular osmolarity

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8
Q

Four types of membrane proteins

A

Linker/anchor

Transporter

Receptors-signalling

Enzymes

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9
Q

Brush border

A

Microvilli boarder

-example of one of mods PMs can have

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10
Q

Cell adherence is possible w/

A

Junctional complexes

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11
Q

Glycocalyx

A

Fuzzy coat on tips of microvilli

Enteric surface coat

Created from several glycosylated molecules at surface of cell

Glycosylation occurs in RER

Consists of glycolipids, glycoproteins, and proteoglycans

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12
Q

What stabilizes microvilli?

A

Actin-cytoplasm surrounds finger like projection of actin bundle

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13
Q

Terminal web

A

Filamentous complex at top of cells where actin of villi terminate

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14
Q

Myosin in microvilli

A

Move material down microvili

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15
Q

Terminal bars

A

identify locations of junctional complex

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16
Q

Microfilaments ar made of…

A

Actin-extend throughout cell

17
Q

Microtubules

A

Extend through cytoplasm

Structural bas of centrioles

Form cilia and mitotic spindles

Made of tubulin

18
Q

Grandular vs Agranular reticulum

A

RER vs SER

19
Q

SEE structure+ function

A

Consists of expensive array of membrane tubules and enclosed cisterns-no ribosomes

Lipid and steroid biosynthesis+metabolize many substances

Also site of storage for divalent ion calcium-site of many heme linked proteins

20
Q

RER

A

Cistern=ribsome membrane bounded space

Start in nucleus, go to RER, go to golgi (sorted for final dentition here)

21
Q

rRNA dyeing

A

bind to costively-charged dyes and produce basophila

22
Q

ER in sec cells

A

Sec cells can be loaded with RER

23
Q

Golgi faces

A

Cis-attached to RER

Medial

Trans-Results in secretory vesicles/can also store secretory vesicles

24
Q

How does transport between goggle cisternae occur

A

vesicle trafficing

25
Q

Constitutive secretion

A

Contiual secretion of proteins

Distinct lack of storage/secretory granules

26
Q

Regulated secretion

A

Large number s of granules accumulate-many sec vesicles

Secretion initiated by external stimuli

27
Q

Early endosome pathway

A

Coated pit to coated vescile, then bind to cell surface receptor
(receptor mediated endocytosis)
-fuse with early endosome

Clathirin-protein that coats

28
Q

What is an endosome

A

Endosomes-distinct organelles consisting of vesicles containing material that has entered cell

  • early endosome-receptors and ligands are separated, and receptors often recycle to surface
  • nutrients routes to lysoeom via late endosome where nutrients can be released from carrier proteins and made averrable to cell
29
Q

Mitochorndira S+F

A

Double membrane-inner membrane has cristae
-many different shapes depends on cell needs

Oxidative phosphorylation to synthesize ATP
-Someting to do with cell death

30
Q

Cristae numbers

A

Depends on energy needs
=more energy=more cristae

some many not even have cristae

31
Q

Mito DNA makes

A

most mitorRNA, mito rtRNA

hydrophobic subunits of enzymes involve din oxidative phosphorylation

Most protein of mito are made by other DNA

32
Q

Lysosomes

A

Seen by a specific stain

Packets of hydrolytic enzymes that become active at acidic pH
-sites of intracellular digestion

Eat external molecules as well as worn-out intracellular organelles

Also can eat entire cells (phagocytosis)

Hard to identify

33
Q

Eosinophil

A

Found in circulating blood and in connective tissue-fight drastic infections
-have crystallline inclsions=HAMBURGER!

34
Q

Peroxisomes

A

Distinct protein complements

  • specific metabolic functions
  • inclusions that are not easily seen by immunocytochem
  • Cryalline core in EM=dense material in matrix

BMS 2 (41 decks)

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