Intro

Question 1

What is histology?

Answer 1

Microscopic anatomical study of biological tissue

Question 2

What is histopathology?

Answer 2

Branch of histology that includes the microscopic identification of diseased tissue

Question 3

What can tissues be classified as?

Answer 3

-Epithelial tissue - cover surfaces, line body cavities, form solid glands
-Connective tissue - produce an ecm, link or support other specialised tissues
-Muscle tissue - cells w contractile properties
-Nervous tissue - cells of brain, spinal cord and nerves

Question 4

What does a light microscope do?

Answer 4

Forms a magnified image by focusing light with glass lenses on a thin tissue section

Question 5

Purpose of condenser lens?

Answer 5

Focuses light from the illumination source onto the specimen

Question 6

What does the objective lens do?

Answer 6

Collects light that passes through the specimen, enlarges and projects an image onto the eyepiece

Question 7

What does the eyepiece/occular lens do?

Answer 7

Further magnifies images and projects onto retina

Question 8

What tissues are called basophilic and what charge dp they have?

Answer 8

Tissues components that stain with basic dyes are called basophilic
-Basophilic dyes have a net positive charge so bind to components of cells/tissues that are negatively charged
e.g DNA and RNA (Binds to phosphate groups of nucleic acids)
-Some polysaccharides (sulphate groups) and some proteins (mucus)

Question 9

What tissue components are referred to as acidophilic and what charge do they have?

Answer 9

Tissue components that stain with acid dyes are referred to as acidophilic
-Dyes have net neg charge and bind to components of tissues that are pos charged
e.g ionised amino groups in proteins

Question 10

Look at histologfical stains table