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Molecular Exam 2 > Intracellular Vesicular Traffic 1 > Flashcards

Intracellular Vesicular Traffic 1 Flashcards

1
Q

vesicular transport mediates a continuous _______ of components between various membrane enclosed compartments

A

exchange

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2
Q

What gives each compartment its full molecular address?

A

combination of markers

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3
Q

________ pathways return molecules to source

A

retrieval

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4
Q

Cells _______ proteins into membrane domains by assembling a special ______ on cytosolic side of the membrane

A

segregate; coat

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5
Q

What are the two major functions of coats?

A

concentrates select proteins in a specialized path for transport
models the vesicle to form a basket like lattice that deforms the membrane and gives shape to the vesicle

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6
Q

What are the types of coated vesicles?

A

COPI
COPII
Clathrin

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7
Q

COPI mediates transport from ______

A

golgi cisternae

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8
Q

COPII mediate transport from ______

A

ER

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9
Q

Clathrin coasted vesicles mediate transport from ______ and ______

A

Golgi apparatus

plasma membrane

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10
Q

Each clathrin subunit has how many polypeptide chains? and what does it form?

A

3 large and 3 small chains

form three leg structure called triskelion

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11
Q

Triskelions assemble into a __________ structure of hexagons and pentagons that form coated pits on the ______ side of membrane

A

basket like

cytosolic

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12
Q

What forms a second layer between the cage and the membrane in clarthrin coated vesicles?

A

adaptor proteins

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13
Q

assembly of the coat protein molecules introduces a ______ in the membrane

A

curvature

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14
Q

After the curvature forms in the membrane, what happens?

A

formation of coated buds

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15
Q

______ proteins bin clathrin and membrane bound cargo receptors

A

adaptor

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16
Q

After vesicle formation is complete, what happens to the clathrin coat?

A

it is lost

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17
Q

What does phosphoinositides do?

A

play a major role in coat assembly, vesicle formation and protein trafficking

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18
Q

Where do phosphoinositides undergo phosphorylation and dephosphorylation? and what does it form?

A

at the 3’, 4’ and 5’ positions

forms various derivatives

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19
Q

Interconversion between various phosphoinositides is highly compartmentalized, _______ specific and ______ specific

A

organelle

domain

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20
Q

Different organelles have unique sets of ________ and ______ to facilitate inter conversion

A

PI kinases

phosphatases

21
Q

When removing the clathrin coat, what protein assembly around the clathrin bud?

A

dynamin

22
Q

What domains does dynamic contain?

A

PIP2 binding domain

GTPase binding domain

23
Q

What does the PIP2 binding domain do?

A

tethers the protein to membrane

24
Q

What does the GTPase binding domain do?

A

regulates rate of vesicle pinching

25
Q

What does pinching do?

A

brings the 2 cytosolic sides of the membrane together and allows them to fuse

26
Q

What protein uses ATP to peel off the clathrin coat?

A

HSP 70

27
Q

What do Rab proteins do?

A

direct vesicle to specific spots on target membrane

28
Q

What do SNARE proteins do?

A

mediate fusion of vesicle with membrane

29
Q

What are Rab proteins actively regulated by?

A

GTPases

30
Q

Each Rab is ______ with one or more membrane compartments of the secretory or endocytic pathway

A

marker

31
Q

Rab is inactive in what form? active?

A

inactive: GDP bound
active: GTP bound

32
Q

What happens when bound to Rab-GDP dissociation inhibitor?

A

keeps inactive GDP bound form, form is soluble

33
Q

Where is Rab1 located?

A

ER and golgi complex

34
Q

Where is Rab3A located?

A

synaptic vesicles, secretory vesicles

35
Q

Where is Rab5 located?

A

early endosomes, plasma membrane, clathrin coated vesicles

36
Q

What do SNARE proteins do?

A

catalyze fusion of vesicles with membranes

37
Q

How many different SNAREs are there?

A

35

38
Q

where are v-SNAREs found?

A

vesicle membrane

39
Q

Where are t-SNAREs found?

A

target membranes

40
Q

When v and t SNAREs interact, what happens?

A

wrap around each other to form a bundle

41
Q

Newly synthesized proteins are packaged into ________ coated transport vesicles.

A

COPII

42
Q

How does quality control occur in transport from ER?

A

incomplete/misfolded proteins are contained in the ER by binding to chaperone proteins such as BiP and calnexin, then proteins degrade

43
Q

What is homotypic fusion?

A

fusion of vesicles from same compartment

44
Q

What is heterotypic fusion?

A

fusion of vesicles from different compartment

45
Q

What are fused structures called?

A

vesicular tubular clusters

46
Q

What are the 2 faces of the golgi?

A

cis (or entry) and trans (or exit)

47
Q

What is the Cis golgi network (CGN)?

A

network of fused vesicular tubular clusters arriving from ER. Proteins and lipids enter golgi via CGN

48
Q

What is the trans golgi network (TGN)?

A

region that allows exit of proteins and lipids to next step fo secretory pathway

49
Q

Golgi plays important role in protein______

A

glycosylation

Molecular Exam 2 (13 decks)

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