instrumentation Flashcards
mass spectrometer
used to analyse blood of race horses for drugs, identify substances
Atomic Absorption Spectroscopy (AAS)
used to analyse water samples for heavy metals (Cd, Hg, Pb)
GC
used with MS in drug testing also blood alcohol levels
HPLC
used to separate less volatile mixtures eg. growth promoters in meat
IR spec
used to identify functional groups + identify drugs
UV spec
quantitative used to find amount of organic substances
eg. drugs
instruments
gas chromatograph mass spectrometer atomic absorption spectrometer high-performance liquid chromatograph infrared spectrometer ultraviolet absorption spectrometer
Gas Chromatograph - principles
different components of a mixture have diff interactions w/ the stationary + mobile phases
Gas Chromatograph - processes
- injection
- transport of sample along column
- separation in column
- detection
Mass Spectrometer - principles
positively charged ions are separated on basis of their relative masses in a magnetic field
Mass Spectrometer - processes
- vaporisation
- ionisation
- acceleration
- separation
- detection
Atomic Absorption Spectrometer - principles
ground state atoms of an element absorb light characteristic of that element. Absorbance is directly proportional to concentration
Atomic Absorption Spectrometer - processes
- Sample soln sprayed into a flame + sample element converted into atoms of that element
- Ground state atoms absorb radiation from source made from element
- Absorbance measured
High-performance liquid chromatograph - principles
Different components of mixture have different tendencies to absorb onto v. fine particles of a solid in the HPLC column
High-performance liquid chromatograph - processes
- injection
- transport of sample along column
- separation in column
- detection