insect cuticle Flashcards

1
Q

what are the roles of the insect cuticle

A
  • protection from predators and dessiccation
  • provides shape and support to soft tissues
  • protection from attack or injury
  • first line of defence against pathogens
  • minimise the loss of body fluids
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2
Q

what are the functions of the cuticle

A
  1. Mechanical protection
  2. Prevents desiccation
  3. Protective barrier against bacteria, viruses, toxins
  4. Location of colours and patterns important in
    behaviour
  5. Lines:
    a) External surface of the body
    b) Tracheae
    c) Anterior and posterior sections of alimentary
    canal
    d) Parts of the reproductive system
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3
Q

how does the insect cuticle support the body and provide points for muscle attachments

A

muscles which act on the exoskeleton are connected to the basal surfcae of epidermal cells through structurs called desmosomes

= muscular forces are transferred through these cells by microtubules running parallel from the bottom to top of the cells where they attatch to tonofilaments which run through the epidermal cells

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4
Q

what is an apodeme

A

refers to the internal ridge or process in the exoskeleton of insects
= serve as attachment sites for muscles providing structural support and facilitating movement

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5
Q

why can insects have more muscles, despite their size, compared to vertebrates

A

the exoskeleton has a larger surface area compared to an endoskseleton with alot more areas for muscle attachement
= having so many areas for muscles to attach allows increased leverage for appendages which is why insects are so much stronger

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5
Q

briefly outline the structure of an insect cuticle

A

consists of both living and non living layers- from outside in
1) epicuticle made up of
- cement layer
- wax layer
- outer epicuticle
- inner epicuticle

2) procuticle made up of
- exocuticle
- endocuticle

3) living epidermal cells

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5
Q

outline the structure of the living layer of the insect cuticle

A

rows of epithelial cells resting on connective tissues- forms basement membrane for the rest of the cuticle to sit
= cells secrete the non-living layers of cuticle during moulting
= other cells are interspersed in these epithelia cells and form sensory structures such as setae and hairs

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6
Q

outline the structure of the non-living layers of the insect cutcile

A

consists of two main layers - thin epicuticle and thicker inner procuticle

1) epicuticle - made up of addition 4 layers
- inner epicuticle = lipoprotein layer
- outer epicuticle
- wax layer = fragile but needed for water retention
- cement layer= protects the wax layer

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7
Q

outline the structure and function of the cement layer

A

can be absent in some insects
= thin layer which is found above the wax layer and produced by dermal glands
= consists of tanned proteins and lipids causing a hard shellac like substance

= protects against abrasion and isnt replaced if it is abraded until the next moult

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8
Q

outline the structure and function of the wax layer

A

varies in composition in different insects
- contains fatty acids, hydrocarbons and alcohols
= lipids are synthesised by oenocytes (specalised cells in the insect body) and transported by pore canals
= wax is transported to the surface of the insect via these pore canals
= change permeability of the cuticle to water

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9
Q

outline the structure and function of pore canals

A

= cytoplasmic extensions of epidermal cells which transport cuticular materials to the surface
= occur in large numbers
= shape probably determined by the orientation of fibres making up lamellae in the endocuticle

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10
Q

what is the inner procuticle made up of

A

consists of two layers
1) Exocuticle = dark, outer layer
2) Endocuticle = pale, inner layer

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11
Q

outline the constituents of the epicuticle

A

the outer epicuticle (3/4 layers) contains no chitin
the outer layer = made up of non-elastic lipids and proteins
the inner layer = made up of tanned lipoproteins

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12
Q

outline the constituents of the procuticle

A

the inner cuticle (2 layers) has chitin
= first secreted as procuticle before the outer is sclerotised exocuticle and the inner is left undifferentiated endocuticle

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13
Q

outline the structure of chitin

A
  • Polymer of N-acetylglucosamine
  • Makes up 20-50% dry weight of
    procuticle
  • Neighbouring chains run in
    opposite directions
  • Held together by H-bonds
  • Form microfibrils (Ø = 2.8 nm)
  • Embedded in protein matrix
    (reinforced concrete)
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14
Q

outline the arrangement of the cuticle

A

each polymer chain of chitin runs in opposite directions to the neighbouring chain and is linked via hydrogen bonds forming microfibrils which gorup to form fibrls which wrap with proteins and assemble forming fibres which assemble into bundles

= these bundles arrange parallel to one another forming a pseudo layer which stack into a helical structure which each layer roated around the normal axis of the cutcile

15
Q

what are the different types of microfibril orientation seen in insect cuticles

A

1) Lamellate cuticles
2) unidirectional cuticle
3) pseudo-orthogonal cuticle

16
Q

outline the microfibril orientation of a lamellate cutcile

A

each layer (lamella) rotates anti-clockwise by a fixed angle forming a helicoidal arrangement
= most insects have a lamallate outer procuticle

17
Q

outline the orientation of microfibrils in unidirectional cuticles

A

lamellate (helical arrangement with alternated microfibres) are alternated with uniformly orientated microfibrils in the same direction

18
Q

outline the orientation of microfibrils in pseudo-orthoganl cutciles

A

lamellate alternated with uniformly oreinated microfibrils in differing directions

19
Q

outline protein as part of the insect cuticle

A

Major constituents of insect cuticle
* Different parts may have different
proteins
* Soft cuticle has more aspartic
acid, glutamic acid, histidine,
lysine and tyrosine
* More hydrophilic than those of
hard cuticles
* Secreted from Golgi apparatus

20
Q

list the different ways the procuticle can change

A

1) remain undifferenciated
2) become a rigid cuticle
3) become a membranous cuticle
4) become an elastic/extensible cuticle
5) undergo calcification

21
Q

outline how the procuticle can remain undifferenciated

A
  • Found in many holometabolous larval
    insects
  • Allows larva to remain extensible and
    facilitates intrastadial growth
  • Permits movement by change in body form
  • Conserves energy as moult numerous
    times so most digested and reabsorbed
22
Q

outline how the procuticle can change to become a rigid cuticle

A

produced as a result of tanning = the extent of tanning/hardness can vary
the procuticle turns to the exocutucle
tanned exocutucle = hard, insoluable, dehydrated, dhrunken

= an irreversible chemical process

23
Q

outline how the procuticle can change to produce a membranous cuticle

A

Flexible membrane
between sclerites
* Procuticle remains
untanned
* Proteins have
different amino acid
composition
* Extent of membrane and
method of articulation
determine degree of movement
* Monocondylic = antennae
* Dicondylic = legs
* Undifferentiated endocuticle
soft, plastic, hydrated, swollen
* Reversible process

24
Q

outline how the procuticle can change to produce an elastic/extensible cuticle

A
  • Resilin is rubber-like protein
    found in elastic hinges of cuticle
  • Amino acids in resilin form crosslinks between protein chains,
    preventing other cross-linking
  • Stretched under tension, stores
    kinetic energy and shows no
    deformation
25
Q

outline how the procuticle can change through calcification

A
  • Minerals = calcite, vaterite and hydroxyapatite
  • Some immature insects - immatures as makes
    cuticle too heavy for flight
  • Replaces tanning in larval and puparial cuticle
    of Musca autumnalis (Face fly)
  • Oothecae of cockroaches and mantids (calcium
    oxalate, or calcium citrate, or calcium
    carbonate)