Inhibitors Flashcards
Binds to the 30s subunit interfering with binding fMet-tRNA; prevents initiation by stopping association with 50s
Streptomycin
binds to 30s preventing aminoacyl-tRNA from entering complex
Tetracycline
Inhibits peptidyl transferase (elongation) in prokaryotes and mitochondria
Chloramphenicol
Binds to 50s, blocking translocation of ribosome
Clindamycin and Erythromycin (pertussis)
Inhibits peptidyl transferase in eukaryotes; Strep. griesua
Cycloheximide
inactivates GTP bound EF-2; interfering with eukaryotic ribosomal translocation
Diptheria Toxin
Bind to 60s of eukaryotes and prevent the entry of aminoacyl-tRNA to complex
Shiga Toxin and Ricin
Premature chain termination in prokaryotes and eukaryotes; elongation inhibitor
Puromycin
Block DNA replication; affect all phases of cell cycle
Alkylating agents
Inhibit topoisomerase II, which gets rid of tangles in DNA
Topoisomerase II inhibitors in S phase (Etoposide)
Inhibit enzymes involved with DNA synthesis
Antimetabolites
Intercalate between bases in DNA to inhibit synthesis
Cytotoxic antibiotics
Inhibit topoisomerase I, which removes supercoils in DNA
Topoisomerase I inhibitors in G2 (Irinotecan)
Arrest cells in mitosis during metaphase
Mitotic inhibitors
Binds and stabilizes microtubules
Taxol
Colchicine (colcemid) and Vinblastine (Vincristine)
Binds tubulin dimers and prevents polymerization
Binds and stabilizes actin (micro) filaments preventing their depolymerization
phalloidin
Cytochalasin
caps filament plus ends; preventing further polymerization
Latrunculin
Binds actin monomers to prevent polymerization
