Inhibition of Enzyme Activity Flashcards
What are the different methods of enzyme inhibitors to reduce enzyme activity?
competitive inhibition
noncompetitive inhibition
uncompetitive inhibition
mixed-type inhibition
What are competitive inhibitors?
molecules that compete with substrate for binding at the active site
How is the Vmax and Km affected by competitive inhibitors?
Vmax has no change
Km will increase
How is the Vmax and Km affected by noncompetitive inhibitors?
Vmax decreases
Km no change
How is the Vmax and Km affected by uncompetitive inhibitors?
Vmax decreases
Km decreases
How is the Vmax and Km affected by mixed-type inhibitors?
Vmax decreases
Km varies
What are noncompetitive inhibitors?
bind to an allosteric site, not the active site
What are uncompetitive inhibitors?
inhibitor that is only able to bind to the enzyme-substrate complex (cannot bind before the substrate has bound), will bind at an allosteric site
What are mixed type inhibitors?
an inhibitor that can bind to either the unoccupied enzyme or the enzyme-substrate complex
What is a Lineweaver-Burk plot?
graphical representation of enzyme kinetics
