Enzyme Kinetics

Question 1

What is enzyme kinetics?

Answer 1

the study of the rate of formation of products from substrates in the presence of an enzyme

Question 2

What is the reaction rate?

Answer 2

V = amount of product formed per unit time, in moles per second (mol/s)

Question 3

What is the reaction rate dependent on?

Answer 3

concentration of the substrate and enzyme

Question 4

What does it mean for an enzyme to be saturated?

Answer 4

Vmax = so much substrate that the active site is continuously occupied and adding more substrate does not increase the rate of the reaction

Question 5

What is the Michaelis constant?

Answer 5

Km = substrate concentration at which the reaction velocity is half of its maximum

Question 6

What does it mean for a substrate to bind cooperatively?

Answer 6

binding of one substrate to one subunit modulates the affinity of other subunits for substrate

Question 7

What are the two types of cooperativity?

Answer 7

positive
negative

Question 8

What is positive cooperativity?

Answer 8

binding of a substrate to one subunit increases the affinity of the other subunits substrate

Question 9

What is negative cooperativity?

Answer 9

binding of a substrate to one subunit reduces the affinity of the other subunits for substrate

Question 10

What type of enzymes does cooperativity not apply to?

Answer 10

catalytic enzymes