Infiltration/Impregnation Flashcards

1
Q

Infiltration is carried out for what two reasons

A
  1. Remove clearing agents from tissue
  2. Fill up cavities and tissue spaces
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2
Q
  • Infiltration method recommended for specimens with large and hollow cavities that tends to collapse
  • Disadvantage: Process is slow
A

Celloidin Infiltration method

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3
Q

Thin celloidin

A

2-4% ; place the specimen for 5-7 days

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4
Q

Medium celloidin

A

4-6% ; 5-7 days

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5
Q

Thick celloidin

A

8-12% ; 3-5 days

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6
Q
  • Most common method of infiltration and makes the process rapid because it uses heat
  • Not suited for fatty tissues
A

Paraffin Wax method

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7
Q

Not usually carried out in the lab; recommended only if dehydration must be avoided
Infiltration method for enzyme ad histochemical studies

A

Gelatin Infiltration Method

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8
Q

Gelatin method will require tissue to be more than

A

2-3 mm thick

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9
Q

The use of __ in gelatin filtration will prevent mold

A

Phenol

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10
Q
  • Celloidin infiltration method recommended for whole eye specimen.
  • Will require use of Gilson’s mixture to store blocks
A

Dry Celloidin method

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11
Q
  • Recommended for bones, teeth, large brain sections and whole organs
  • Celloidin infiltration method for bones, brain and teeth specimens
  • Will require 70-80% alcohol to store blocks
A

Wet celloidin metho

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12
Q

Another form of celloidin that is soluble in ether and alcohol

A

(Low Viscosity Nitrocellulose) or LVN

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13
Q
  • Has lower viscosity therefore it can be used in high concentration and can penetrate tissue rapid
  • Much preferred than the ordinary celloidin for both impregnation and embedding
A

Nitrocellulose Method

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14
Q

Produces harder tissue block and allows cutting of thinner sections

A

Nitrocellulose Method

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15
Q

Disadvantage of LVN

A

they have the tendency to crack

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16
Q

Remedy for cracks made by LVN

A

add pasticizers (Oleum ricini or castor oil)

17
Q

Most common infiltrating agent

A

Paraffin Wax

18
Q

Melting point of Paraffin wax

A

56degC

19
Q

Paraffin infiltration that uses heat so we need to utilize an oven

A

Manual Method

20
Q

temperature range of paraffin oven when in used

A

55-60degC or approx. 2-5degC higher than the wax melting ping

21
Q

How many changes of wax is required in manual method?

A

4 changes

22
Q

uses autotechnicon that requires 2-3 changes of wax

A

Automatic Method

23
Q

Infiltration under negative atmospheric pressure inside the oven

A

Vacuum method

24
Q

T/F: In vacuum method, tissue processing time is reduced because tissues are subjected to very high temperature

A

TRUE

25
Q

Substitute for paraffin wax recommended for bones and brain specimens

A

Paraplast

26
Q

Melting point of paraplast

A

56-57degC

27
Q

Substitute for paraffin wax that is water soluble and is recommended for enzyme histochemical studies

A

Carbowax

28
Q

An polyethylene glycol containing18 or more carbon atoms; appears solid at room temperature

A

Carbowax

29
Q

Substitute for paraffin wax used for eye specimens

A

Bioloid

30
Q
  • Substitute for paraffin wax that will require heavy duty type of microtome
  • Harder than paraffin wax
A

Ester Wax