Clearing/Dealcoholization

Question 1

Removal of alcohol from tissue acquired from dehydration process

Answer 1

Clearing/Dealcoholization

Question 2

• Routinely used clearing agent but not for nervous tissue and lymph node
• Can become milky after placing an incompletely dehydrated tissue on it

Answer 2

Xylene

Question 3

Clearing agent that can damage the bone marrow and may cause aplastic anemia

Answer 3

Benzene

Question 4

Clearing agents that can be easily removed from tissue > shortened infiltration time

Answer 4

Benzene and Xylene

Question 5

Can be used as xylene and benzene substitute

Answer 5

Toluene

Question 6

• Toxic to liver
• Recommended for skin, fibroid and decalcified tissues for tough tissue, nervous tissues, lymph nodes, embryos
• Clearing agent that will not make tissues transparent
• Clearing agent that can attack rubber seal used in vacuum impregnation

Answer 6

Chloroform

Question 7

Has the same property as chloroform

Answer 7

Carbon tetrachloride

Question 8

• Can become milky because of prolonged storage
• Clearing agent for CNS tissues & cytological studies, an extremely slow clearing agent

Answer 8

Cedarwood oil

Question 9

• Can be used for dense tissues like uterus
• Agents difficult to remove form tissue > prolong infiltration time

Answer 9

Chloroform and Cedarwood Oil

Question 10

For clearing insects, embryos and other delicate specimens

Answer 10

Aniline oil

Question 11

Tissue become adulterated when used

Answer 11

Clove oil

Question 12

Terpineol

Answer 12

Recommended for eye specimen

Question 13

Clearing agent obtain from citrus fruit with faint pleasant odor

Answer 13

Limonene

Question 14

For Double Embedding (Slow Acting)

Answer 14

1. Methyl salicylate
2. Methyl benzoate
3. Amyl acetate

Question 15

Steps in tissue processing that will require complete clearing of tissue

Answer 15

Infiltration and Mounting