Embedding/Casting/Blocking Flashcards
Placing infiltrated tissue in a mold containing an embedding medium which is allowed to solidify
Embedding
Other names for Embedding
Casting or block
Product of embedding
Tissue block
Requirements for embedding
Embedding mold, medium and infiltrated tissue
The most common embedding medium
Paraffin wax
A disposable embedding mold that will require smearing of inner mold with glycerine
Plastic Ice trays
- A embedding mold consisting of a series of interlocking plates resting on a flat metal base
- Can embed more than 1 specimen at the same time
Compound Embedding unit
Disposable mold that gives perfect blocks without trimming
Peel Away Molds
- Process of infiltrating tissue with celloidin and embedding with paraffin
- Uses two different medium
Double Embedding
- Embedding mold consisting of L-shaped strips made of heavy brass
- Not often used in the lab because it needs to be assemble
Leuckhart’s
Advantage of using Leuckhart’s
can produce different block sizes that depends on you
Provides superior result in light microscopic studies (eg. Undercalcified bone)
Plastic (Resins) Embedding
Fastest epoxy resin
Spurr
plastic resin originally introduced for EM
Polyester
extremely hydrophilic
Polyglycl Methacrylate/GMA