Dehydration Flashcards
Step in tissue processing that involves removal of intra and extracellular water from tissue
Dehydration
Commonly used in dehydration, fast acting and nontoxic
Alcohol
Concentration of alcohol must be in
Ascending or increasing concentration
What will happen if the concentration is in decreasing concentration?
Tissue will be damaged
Most commonly used alcohol for dehydration process
Ethyl alcohol
Alcohol for plant and animal micro technique
Butyl alcohol
Found in alcohol bath and act as a tissue softener (for nails etc)
Phenol
For dehydrating sections and smears
Triethyl phosphate
- Toxic by ingestion, inhalation and by skin contact
- Highly corrosive on skin
- Toxic to reproductive, fetal and urinary system
- Decomposes when expose to sunlight
Cellosolve
Other name of Cellosolve
Ethylene Glycol Monoethyl Ether
- Can dehydrate and clear tissue at the same time but not commonly used
- Dehydrating agent whose main disadvantage is its being toxic
Dioxane
Other name of Dioxane
Diethylene Dioxide
- Dehydrating agent that is both a skin and eye irritant
- Has an offensive odor – therefore, the room should be well ventilated
Tetrahydrofuran (THF)