Infiltration/Impregnation Flashcards
Purpose of infiltration
To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting
Purpose of infiltration
To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting
Infiltration Methods:
- Manual
- Automatic
- Vacuum
Paraffin wax substitute: (4)
- Paraplast
- Bioloid
- Carbowax
- Ester Wax
Infiltration under negative atmospheric pressure inside the oven
Vacuum
Most rapid method as it subject tissues to a very high temperature
that facilitates removal of transition solvents and prolongs life of wax
by preventing solvent contamination
Vacuum
Uses autotechnicon which requires 3-4 changes of wax
Automatic
Temperature range for manual infiltration
55-60°C
Approximate temperature when using manual infiltration
2-5°C higher than the wax melting point
Recommended for bones
Paraplast
Melting point of Paraplast
56-57°C
Substitute for paraffin wax used for eye specimens
Bioloid
Paraffin substitute that is water soluble
Carbowax
What is carbowax?
A polyethylene glycol that has 18 or more carbon atoms
Appears solid at room temperature
Carbowax