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histoopaathh > Decalcification > Flashcards

Decalcification Flashcards

1
Q

Factors that affect decalcification time: (4)

A
  1. Degree of mineralization
  2. Volume of decalcifying agent
  3. Temperature
  4. Application of heat/agitation
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2
Q

Ratio of agent to tissue volume

A

20:1

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3
Q

Temperature when decalcifying specimen

A

18-20°C

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4
Q

Method to measure the extent of decalcification: (4)

A
  1. Mechanical/Physical
  2. Radiological/Xray
  3. Bubble test
  4. Chemical/Calcium oxalate
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5
Q

Method of testing for the completeness of decalcification done by probing tissue with a needle

A

Mechanical/Physical

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6
Q

Most reliable method of testing for the completeness of decalcification

A

Radiological/Xray

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7
Q

This method of testing uses calcium carbonate

A

Bubble test

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8
Q

Presence of this indicates incomplete decalcification

A

Presence of bubbles

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9
Q

Methods of decalcification: (4)

A
  1. Use of chelating agent
  2. Electrolytic method
  3. Use of acid
  4. Ion Exchange Resin
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10
Q

Potent anticoagulant but weak decalcifying agent

A

EDTA

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11
Q

Binds with calcium to form weakly dissociated complexes

A

EDTA

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12
Q

An excellent bone decalcifier for EM and enzyme histochemistry

A

EDTA

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13
Q

Disadvantage of EDTA

A

Inactivates alkaline phosphatase

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14
Q

Most rapid decalcification method that involves attraction if positively charged calcium ions with negatively charged electrodes

A

Electrolytic method

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15
Q

Use of hydrochloric acid

A

Surface decalcification of blocks

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16
Q

Use of Perenyi’s fluid

A

Decalcifying agent and tissue softener

17
Q

Most commonly used decalcifying agent

A

5-10% Nitric Acid

18
Q

Use of 5-10% Nitric Acid

A

Recommended for urgent biopsies, needle and small biopsies

19
Q

Most rapid nitric containing decalcifying agent

A

Fluoroglucin Nitric

20
Q

Duration of Ion Exchange Resin

A

14 days or longer

21
Q

Tissue softener’s that is utilized if the tissue is still hard after decalcification

A

Molliflex - Perenyi’s

histoopaathh (7 decks)

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