Clearing/Dealcoholization Flashcards
This is required before infiltration and mounting
Complete clearing of tissues
Clearing agents: (10)
- Xylene
- Benzene
- Toluene
- Chloroform
- Cedar wood oil
- Carbon Tetrachloride
- Aniline oil
- Clove Oil
- Terpineol
- Limonene
Disadvantages of Chloroform: (4)
- Toxic to liver
- Doesn’t make the tissues transparent
- Difficult to remove from tissues (together w/Cedar Wood Oil)
- It can attack rubber seal used in vacuum impregnation
Can be used as xylene and benzene substitute
Toluene
Clearing agent that can damage the bone marrow and may cause aplastic anemia on prolonged use
Benzene
Can be easily removed from tissues (together w/xylene)
Benzene
Can become milky after placing an incompletely dehydrated tissue in it
Xylene
Can be easily removed from tissues (together w/benzene)
Xylene
Not for nervous tissues and lymph nodes
Xylene
Routinely used clearing agent
Xylene
For tough tissues, nervous tissues, lymph nodes, embryos
Chloroform
Recommended for skin, fibroid, and decalcified tissues
Chloroform
Can be used for dense tissues like uterus (together with/Cedar Wood Oil)
Chloroform
Clearing agent for CNS tissues and cytological studies
Cedar wood oil
Can be used for dense tissues like uterus (together w/CF)
Cedarwood oil
Can become milky because of prolonged storage
Cedarwood oil
An extremely slow clearing agent
Cedarwood oil
Difficult to remove from tissues (together w/CF)
Cedarwood oil
SAme property as chloroform
Carbon tetrachloride
For clearing insects, embryos, and other delicate specimens
Aniline oil
When used, tissues become adulterated
Clove oil
Recommended for eye specimens
Terpineol
Clearing agent obtained from citrus fruit, With faint pleasant odor
Limonene
For double embedding which are slow acting agents : (3)
Methyl salicylate
Methyl benzoate
Amyl acetate
