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histoopaathh > Clearing/Dealcoholization > Flashcards

Clearing/Dealcoholization Flashcards

1
Q

This is required before infiltration and mounting

A

Complete clearing of tissues

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2
Q

Clearing agents: (10)

A
  1. Xylene
  2. Benzene
  3. Toluene
  4. Chloroform
  5. Cedar wood oil
  6. Carbon Tetrachloride
  7. Aniline oil
  8. Clove Oil
  9. Terpineol
  10. Limonene
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3
Q

Disadvantages of Chloroform: (4)

A
  • Toxic to liver
  • Doesn’t make the tissues transparent
  • Difficult to remove from tissues (together w/Cedar Wood Oil)
  • It can attack rubber seal used in vacuum impregnation
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4
Q

Can be used as xylene and benzene substitute

A

Toluene

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5
Q

Clearing agent that can damage the bone marrow and may cause aplastic anemia on prolonged use

A

Benzene

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6
Q

Can be easily removed from tissues (together w/xylene)

A

Benzene

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7
Q

Can become milky after placing an incompletely dehydrated tissue in it

A

Xylene

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8
Q

Can be easily removed from tissues (together w/benzene)

A

Xylene

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9
Q

Not for nervous tissues and lymph nodes

A

Xylene

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10
Q

Routinely used clearing agent

A

Xylene

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11
Q

For tough tissues, nervous tissues, lymph nodes, embryos

A

Chloroform

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12
Q

Recommended for skin, fibroid, and decalcified tissues

A

Chloroform

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13
Q

Can be used for dense tissues like uterus (together with/Cedar Wood Oil)

A

Chloroform

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14
Q

Clearing agent for CNS tissues and cytological studies

A

Cedar wood oil

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15
Q

Can be used for dense tissues like uterus (together w/CF)

A

Cedarwood oil

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16
Q

Can become milky because of prolonged storage

A

Cedarwood oil

17
Q

An extremely slow clearing agent

A

Cedarwood oil

18
Q

Difficult to remove from tissues (together w/CF)

A

Cedarwood oil

19
Q

SAme property as chloroform

A

Carbon tetrachloride

20
Q

For clearing insects, embryos, and other delicate specimens

A

Aniline oil

21
Q

When used, tissues become adulterated

A

Clove oil

22
Q

Recommended for eye specimens

A

Terpineol

23
Q

Clearing agent obtained from citrus fruit, With faint pleasant odor

A

Limonene

24
Q

For double embedding which are slow acting agents : (3)

A

Methyl salicylate
Methyl benzoate
Amyl acetate

histoopaathh (7 decks)

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