Infiltration Flashcards
Why is there a need to carry out infiltration/ impregnation?
Remove clearing agents from tissue; Fill up cavities and tissue spaces
Why do tissue spaces and cavities need to be filled up?
To give the tissue a firm consistency and facilitate tissues to be easily cut in thin slices.
What are the three infiltration method?
Celloidin Infiltration method, Paraffin, Gelatin Infiltration method
Infiltration method recommended for specimens with large and hollow cavities that tends to collapse.
Celloidin Infiltration method
Disadvantage when using Celloidin Infiltration method.
Process is slow
Thin: _______%; place the specimen for _______.
2-4%; 5-7 days
Medium: _______%; place the specimen for _______.
4-6%; 5-7 days
Thick: _______%; place the specimen for _______.
8-12%; 3-5 days
Most common method of infiltration and makes the process rapid because it uses heat.
Paraffin
Method of infiltration that is not suited for fatty tissues.
Paraffin
Not usually carried out in the lab and is only recommended if
dehydration must be avoided.
Gelatin Infiltration method
Infiltration method for enzyme and histochemical studies
Gelatin Infiltration method
Required thickness when using gelatin infiltration method.
Not to be more than 2-3
Use of ____ In gelatin in filtration will prevent molds.
Phenol
3 types of celloidin impregnation.
Dry celloidin Method, Wet celloidin Method, Nitrocellulose Method (Low Viscosity Nitrocellulose)
Celloidin infiltration method recommended for whole eye specimens.
Dry celloidin Method
Using the Dry celloidin Method will require use of _________ to store blocks.
Gilson’s mixture
Recommended for bones, teeth, large brain sections and whole organs.
Wet celloidin Method
Wet celloidin Method will require what percentage of alcohol to store blocks?
70-70%
Form of celloidin that is soluble in ether and alcohol.
Nitrocellulose Method (Low Viscosity Nitrocellulose)
Has lower viscosity therefore it can be used in high concentration and can penetrate tissue rapidly.
Nitrocellulose Method (Low Viscosity Nitrocellulose)
Much preferred than the ordinary celloidin for both impregnation and embedding.
Nitrocellulose Method (Low Viscosity Nitrocellulose)
Produces harder tissue block and allows cutting of thinner sections.
Nitrocellulose Method (Low Viscosity Nitrocellulose)
Disadvantage of Nitrocellulose Method (Low Viscosity Nitrocellulose).
They have the tendency to crack
