Clearing Flashcards
Removal of alcohol from tissue acquired from dehydration process.
Clearing/ dealcoholization
True or False. Many reagents we use for dealcoholization process can make tissues transparent thus it is also called clearing; but not all can make tissue transparent.
True
Routinely used clearing agent but not for nervous tissue and lymph node.
Xylene
Can become milky after placing an incompletely dehydrated tissue on it.
Xylene
Clearing agent that can damage the bone marrow and may cause aplastic anemia.
Benzene
What are the 2 clearing agents that can be easily removed from tissues causing shortened infiltration time.
Benzene and Xylene
Can be used as xylene and benzene substitute.
Toluene
Clearing agent that is toxic to liver.
Chloroform
Clearing agent that is recommended for skin, fibroid and decalcified tissues for tough tissue, nervous tissues, lymph nodes, embryos.
Chloroform
Clearing agent that will not make tissues transparent.
Chloroform
Clearing agent that can attack rubber seal used in vacuum
Chloroform
Clearing agent that has the same property as chloroform.
Carbon tetrachloride
Clearing agent that can become milky because of prolonged storage.
Cedarwood Oil
Clearing agent for CNS tissues & cytological studies, an extremely slow clearing agent.
Cedarwood Oil
2 clearing agents that can be used for dense tissues like uterus.
Chloroform and Cedarwood Oil
True or False. Clearing agents that are difficult to remove from tissue accelerates infiltration time.
False. It prolongs infiltration time.
For clearing insects, embryos and other delicate specimens.
Aniline Oil
When this clearing agent is used, tissue becomes adulterated.
Clove Oil
Recommended for eye specimen.
Terpineol
Clearing agent obtained from citrus fruit with a faint pleasant odor.
Limonene
What are the steps in tissue processing that will require complete clearing of tissues?
Infiltration and mounting
