Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histopath Midterm > Decalcification > Flashcards

Decalcification Flashcards

1
Q

Step in tissue processing that involves removal of calcium (e.g. bones, teeth, etc.).

A

Decalcification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

True or False. Decalcification is a routine procedure.

A

False, not all specimens will undergo decalcification because it is not a routine step

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Why is decalcification done?

A

For specimens with calcium and other salts, they need to undergo decalcification first to soften hard tissues to facilitate easy cutting.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the main purpose of decalcification?

A

Soften hard tissue to facilitate easy cutting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

True or False. Calcified tissue may damage disposable blades and microtome knives.

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What will you do if decalcification is done but the the tissue is still hard?

A

Use tissue softeners like Molliflex-Pereyni’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How long does decalcification take to be done?

A

24 to 48 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Temperature range for decalcification (room temperature).

A

18-30degC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Ratio of decalcifying agent to tissue volume.

A

20:1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What are the factors that affect decalcification time?

A

Degree of mineralization, Concentration and volume of the decalcifying agent, Heating & agitation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

True or False. If calcium content is high or heavy-mineralized, the process will be shorter.

A

False, it will be longer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

If a specimen is bone from an osteoporotic patient, it will have _________(low/high) calcium content, so the process will be _________ (shorter/longer).

A

Low; shorter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Increasing the concentration and volume of the decalcifying agent, the __________(shorter/longer) the decalcification time.

A

Longer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The ratio of decalcifying agent to tissue is ______.

A

20:1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Heating and agitation _______ (accelerates/ retards) the process.

A

Accelerates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Temperature required when performing decalcification.

A

18-30 degrees Celsius (room temperature)

17
Q

True or False. Decalcification can be done through an autotech.

A

False, there is no special equipment to perform decalcification, it must be done manually

18
Q

What are the 4 methods of decalcification?

A

Use of acids, Use of chelating agent, Ion Exchange Resin, Electric Ionization

19
Q

Decalcification method that is commonly used in the laboratory.

A

Use of acids

20
Q

Example of chelating agent that can be used to decalcify.

A

EDTA

21
Q

How long does it take to decalcify when using Ion Exchange Resin method?

A

1-14 days or longer

22
Q

Decalcification method that is not suited for urgent biopsies

A

Ion exchange resin

23
Q

Considered as the most rapid method of decalcification because it is thru electric current.

A

Electric Ionization

24
Q

Enumerate the methods to measure the extent of decalcification.

A

Xray/Radiologic, Mechanical/Physical, Bubble test, Chemical Method

25
Q

Most reliable and accurate method of detecting the extent of decalcification.

A

Xray/Radiologic

26
Q

Can detect the smallest calcium.

A

Xray/Radiologic

27
Q

Done by bending the tissue, probing or pricking the tissue with a needle.

A

Mechanical/Physical

28
Q

Method of testing for the completeness of decalcification that uses calcium carbonate.

A

Bubble test

29
Q

What reagent is being used in bubble test to detect the completeness of decalcification?

A

Calcium carbonate

30
Q

What does the presence of tiny bubbles signify when CaCO3 is added after decalcification?

A

specimen is not yet decalcified (EXTEND DECALCIFICATION)

31
Q

Method of measuring completeness of decalcification by using 5mL or 0.5mL decalcifying agent.

A

Chemical method

32
Q

In chemical method of checking the completeness of decalcification, how is the acidic decalcification agent be converted into alkaline?

A

By adding strong ammonia.

33
Q

What result signals that decalcification is not yet done when chemical method is utilized?

A

Turbidity

Histopath Midterm (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions