In situ DNA hybridization

Question 1

In situ hybridisation of nucleic acids

Answer 1

In situhybridization is a technique for localizing specific nucleic acid sequences within fixed tissues, cells or chromosomes.

Question 2

• In situ DNA hybridisation

Answer 2

\: Presence and localisation/organisation of 
				DNA sequence (gene/genome

Question 3

What occurs in situ DNA hybridisation ?

Answer 3

in situ hybridization (ISH) is used to map and order genes and other DNA and RNA sequences to their location on chromosomes and within nuclei.

The technique is based on the principle that double-stranded DNA denatures on heating to single-stranded DNA.

Question 4

What are used to enable their detection ?

Answer 4

*stretches of single-strandedDNAused to detect the presence of complementary nucleic acid sequences (target sequences) by hybridisation.

DNA probesare labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.