DNA replication

Question 1

Steps of the DNA replication

Answer 1

1. the two strands of the helix have to be separated, to allow the binding of the replication machinery. This occurs through the binding of the enzyme DNA helicase.
2. DNA helicase is able to detach the strands by splitting the hydrogen bonds between the bases. DNA helicases act at specific regions of the DNA called origins of replication. The unwinding of the two strands by DNA helicase creates a replication fork, a Y-shaped structure where each prong is a single strand of DNA. Which helicase moves along the strands, creating a replication bubble.
3. The separated strands provide a template for synthesising a new strand. Proteins called single-strand binding proteins coat the separated strands keeping them from re-forming a double helix.