Important Flashcards
1
Q
What method did we use to separate out our sample into RNA, DNA and protein?
A
acid phenol guanidine thiocyanate method
2
Q
What are the two vectors used
A
pcHI10
PCMV sport
3
Q
What are the components of a pCHI10 vector
A
Sv40 promoter which drives expression of B galactosidase gene
4
Q
What are the components of the PCMV sport plasmid
A
CMV promoter
B galactosidade gene
Poly A
5
Q
Dye used to count cells
A
Trypan blue