Important Flashcards

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1
Q

What method did we use to separate out our sample into RNA, DNA and protein?

A

acid phenol guanidine thiocyanate method

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2
Q

What are the two vectors used

A

pcHI10

PCMV sport

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3
Q

What are the components of a pCHI10 vector

A

Sv40 promoter which drives expression of B galactosidase gene

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4
Q

What are the components of the PCMV sport plasmid

A

CMV promoter

B galactosidade gene

Poly A

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5
Q

Dye used to count cells

A

Trypan blue

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