Immunophenotyping

Question 0

Myeloid differentiation

Answer 0

First to appear is cD33, then cd 13, 14,117

Question 1

Uncommitted hematopoietic progenitor cells

Answer 1

CD34+

Question 2

Erythroid maturation

Answer 2

Appearance of cd 71 and cd 235a, loss of cd 33

Question 3

Megakaryocyte differentiation

Answer 3

Cd 41 (glycoprotein IIb) and cd 61 (glycoprotein 2 b-3a)

Question 4

NK cell differentiation

Answer 4

CD 56, 57

Question 5

Monocyte differentiation

Answer 5

Cd 11, 14, 15, 64

Question 6

TdT and HLA DR

Answer 6

Expressed on b and t lymphocytes

Question 7

B cell maturation

Answer 7

Cd 10, 19, 20

Question 8

T cell maturation

Answer 8

Cd 3, 4, 7, 8

Question 9

CLL

Answer 9

CD 20, 5, 23

Question 10

Mantle cell

Answer 10

CD 5, 19, 20, fmc7 positive
CD 23 NEGATIVE
t (11;14) Cyclin d 1

Question 11

Marginal zone lymphoma

Answer 11

CD 19, 20, 43 positive

CD 5, 10 Negative

Question 12

Classic Hodgkin lymphoma

Answer 12

CD 15, 30 positive

CD 20, 45 negative

Question 13

Nodular lymphocyte predominant Hodgkin lymphoma

Answer 13

CD 15, 30 NEGATIVE

CD 20, 45 Positive

Question 14

Follicular lymphoma

Answer 14

CD 10, 19, 20 positive
CD 5, 24 negative
t (14;18), bcl2

Question 15

Hairy Cell leukemia

Answer 15

Cd 20, 35, 103 Positive

If pancytopenia and cd 103 positive, think hairy cell

Question 16

Lymphoplasmacytic lymphoma

Answer 16

CD 20, 38, 138 positive with light chain restricted plasma cells

Question 17

Multiple myeloma

Answer 17

Cd 20 NEGATIVE

CD 56, 38, 138 positive with light chain restricted plasma cells

Question 18

Acute B cell lymphoblastic leukemia

Answer 18

TdT positive
Cd 10, 19, 20, 22, 45, 79a Positive
Cd 3 negative

Question 19

Precursor B cell ALL

Answer 19

Cd 10, 19, 34 positive

Question 20

Mature B ALL (burkitts)

Answer 20

CD 10, 19, 20, 22, 25 and surface immunoglobulin positive

Question 21

T cell prolymphocytic leukemia (PLL)

Answer 21

CD 3, 7 56 positive

Question 22

T cell large granular leukemia

Answer 22

Cd 3, 7, 8, 16 positive

Question 23

NK leukemia

Answer 23

Cd 7, 56 positive

Question 24

Peripheral T cell lymphoma

Answer 24

Cd 3, 4, 4, 7, 30 positive

Question 25

Angioimmunoblastic lymphoma

Answer 25

Cd 3, 5, 7, 56 positive

Cd 30 negative

Question 26

Anaplastic large T cell lymphoma

Answer 26

Cd 3, 5 and 7 +/-, cd 4 +, cd 30 +, cd 56 negative
t(2;5) ALK
ALK positive has better prognosis than negative

Question 27

Bernard soulier syndrome

Answer 27

LARGE platelets
Normal pt ptt
Prolonged bleeding time
Decrease in GP1b receptor 
ABNORMAL AGGREGATION TO RISTOCETIN, but normal to collagen and adp

Question 28

Glanzmans thrombaathenia

Answer 28

NORMAL appearing platelets
Normal pt ptt vwf
Prolonged bleeding time 
Decrease in GP2b3a receptor
ABNORMAL AGGREGATION TO Collagen and adp, but normal to ristocetin

Question 29

Congenital afibrinogenemia

Answer 29

Same platelet aggregation as glanzmans but ptt ptand thrombin time prolonged

Question 30

Grey platelet syndrome

Answer 30

Alpha granule deficiency

Abnormal aggregation to collagen

Question 31

Dense granule deficiency

Answer 31

Puerto Rican albinism
HSP1 gene
Low platelet ADP
Second wave of aggregation in response to ADP and epinephrine absent

Question 32

Quebec platelet disorder

Answer 32

Normal coags
Abnormal aggregation with epinephrine
Due to increased urokinase plasminogen activator

Question 33

Chediak higashi syndrome

Answer 33

Albinism which low IQ, nystagmus and hepatosplenomegaly
Pancytopenia
LYST gene defect
Lymphohistiocytic infiltration of all organ systems