Immunology Flashcards

1
Q

What is the most common subclass of IgG?
Which IgG subclass best recognizes polysaccharide antigens?

A

IgG1 = most common subclass (66%); IgG2 = 23%, IgG3 = 7%, IgG4 = 4%

All subclasses involved important for defence against bacteria and viruses via neutralization (toxins + viruses, opsonisation, complement activation, NK cells).

IgG2 best recognizes polysaccharide antigens.

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2
Q

What is the name of the receptor that IgE binds? Which cells is it found on?

A

FC epsilon receptor 1

Mast cells, basophils, eosinophils

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3
Q

What is the function of IgA?

A

Found on mucosal surfaces.

Inhibits pathogenic colonization of mucosal surgaces, tissues, binds to mucus and traps surfaces, inhibits penetration of bacterial toxins

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4
Q

What is the most common immunglobulin isotype?

A

IgA

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5
Q

Complement studies in acquired vs hereditary angioedema?

A

Acquired:
low C1q
Low-normal C1INH
Low = type 1, normal = type 2

HAE 1:
normal C1q
low C1INH

HAE2:
normal C1q
normal C1INH, abnormal functional studies/protein

HAE3: estrogen related

All - low C4, C2, normal C3

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6
Q

Negative acute phase proteins (decrease in inflammation)

A

ATTTRA
albumin
transferrin
transthyretin
transcortin
retinol-binding protein
antithrombin

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7
Q

Acute phase proteins (increase in inflammation)?
Which cytokines stimulate their release/formation?

A

Amyloid A
Alpha 2-macroglobulin
C-reactive protein
Ceruloplasmin
Complement factors
Ferritin
Mannose-binding protein
Haptoglobin
Serpins

Inflammatory interleukins - IL-1, IL-6, IL-8, and TNF-α, released from neutrophils, granulocytes and macrophages .

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8
Q

HLA associated with:

  • RA
  • SLE
  • Ank spond
A

HLA-DR3 = SLE, myasthenia, graves, T1DM, addisons

HLA-DR4 = RA

HLA-B27 = ank spond

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9
Q

Th1 cells:
- differentiate under influence of which cytokines?
- what cytokines do they release?
- what is their major role?

A

Differentiate under influence of IFN-y, IL-12
Secrete IFN-Y, TNF-a
Role - defense against intracellular infections (inc. TB, mycobacteria)

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10
Q

Th17 cells:
- differentiate under influence of which cytokines?
- what cytokines do they release?
- what is their major role?

A

Differentiate under influence of TGF-b, IL-6, IL-1 (reinforced by IL-23)
Secrete IL-17, IL-22
Role - defense against fungi + staph; pro-inflammatory (sitmulate release of IL-16, TNF, G-CSF, GM-CSF); role in psoriasis; recruitment of neutrophils

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11
Q

Th2 cells
- differentiate under influence of which cytokines?
- what cytokines do they release?
- what is their major role?

A

Differentiate under influence of IL-4
Secrete IL-4, IL-5, IL-13 (allergy cytokines)
Role - allegy; atopic responses; defence against helminths

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12
Q

Treg cells
- differentiate under influence of which cytokines?
- what cytokines do they release?
- what is their major role?

A

Differentiate under influence of TGF-beta
Secrete IL-10, TGF-Beta
Role - immune regulation (inhbitory)

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13
Q

T follicular cells
- what cytokines do they release?
- what is their major role?

A

Secrete IL-21
Role - B cell help (class switching, affinity mautiation)

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14
Q

Which cell surface protein does memory T lymphocytes express?

A

CD45Ro

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15
Q

Major roles of CRP

A

Promotes recognition and elimination of pathogens (can bind to Fc receptors on phagocytic cells)

Enhances clearance of necrotic apoptotic cells

Complement system activation via C1q binding (binds to phosphocholine residues on bacterial polysaccharides)

Induction of inflammatory cytokines and tissue factor

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16
Q

Measurement of classical pathway

Low levels?

A

CH50 - lyse sheep RBC coated with IgM (% lyzed)

Low in immune complex disease along with C3 + C4 i.e. SLE flare, post strep GN

17
Q

Measurement of alternative pathway

Low levels?

A

AH50 - measurement of non-IgM sensitized cells (% lyzed)

Low in C7 deficiency, factor H deficiency, properdin deficiency and C3 deficiency

18
Q

Target of dupilumab.

Indications?

A

Anti-IL-4/13

Moderate severe allergic/eosinophilic Asthma
Moderate severe atopic dermatitis

19
Q

Deficiency C3 disease association?

A

recurrent bacterial/pyogenic infections

20
Q

Deficiency in C5-C9

A

MAC - Neisseria species, eg neisseria meningitidis.

21
Q

ART Avoid in Pregnancy, buffalo hump?

A

Efavirenz, Ritonavir

22
Q

ART pancreatitis/peripheral neuropathy

A

Didanosine (pancreatitis), Stavudine, Zalcitabine

23
Q

ART renal colic

A

Indinavir, atazanavir

24
Q

HLA-B5701

A

Abacavir hypersensitivty

25
Q

ART increase risk of AMI

A

PI - navir
Lopinavir, Ritonavir, Abacavir

26
Q

ART Hyperbili

A

Atazanavir

27
Q

FOXP3 gene mutation causea

A

IPEX - immune dysfunction, polyendocrinopathy, enteropthy, X-linked.

28
Q

AIRE mutation

A

Autoimmune Polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED)