Immunologic And Serologic Assays Flashcards
_____ may obstruct precipitin bands or may appear to be a precipitin band.
Lipemia or hemolysis
Double diffusion in Two dimensions.
Ouchterlony’s Method
This particular reaction pattern is seen when two Antigens are identical and produce similar precipitin lines. They appear to be fused
Reaction of identity
Where the Ag and Ab form a precipitate is known as what?
Equivalence point
Two separate precipitin lines form and completely cross one another due to separate interactions of the Ab solution with Ag
Reaction or non-identity
The most common support medium for serum protein electrophoresis
Cellulose acetate
Positive pole
Anode
Negative pole
Cathode
The smallest and most abundant serum protein
Albumin
Contains acute phase
Alpha 1 and Alpha 2
Contains Ig A as well as transferrin, beta lipoprotein, and c3
Beta region
Contains Ig M and Ig G
Gamma region
These two malignant disease are characterized by uncontrolled proliferation of a single clone of plasma cells and exhibit a great increase in proteins in the alpha 2 to gamma regions due to increased production of antibody of one class
Multiple myeloma or macroglobulinemia
A condition associated with infection or autoimmune diseases and exhibits an elevated gamma globulin due to increased Ig G production. In increase in all antibody classes may occur with this condition
Polyclonal hypergammaglobulinemia
Levels of gamma globulin may be between 10-50% of normal due to a number of predisposing factors such as a genetically determined defect in antibody synthesis, decreased synthesis of normal antibody myelomas, excessive loss of antibodies.
Hypogammaglobulinemia
