Immunoglobulins

Question 1

Light chains can be [1] (list all subtypes)

Heavy chains can be [2] (list all subtypes)

Answer 1

[1] lambda or kappa

[2] gamma, alpha, mu, delta, or epsilon

Question 2

The three highest concentration immunoglobulins are:

[1] > [2] > [3]

Answer 2

[1] IgG
[2] IgA (dimer)
[3] IgM (pentamer)

Question 3

[1] - most abundant in serum
[2] - mucosal secretions
[3] - produced early in immune response
[4] - function unknown
[5] - symptoms of allergies; parasitic infections

Answer 3

[1] IgG
[2] IgA
[3] IgM
[4] IgD
[5] IgE

Question 4

The immunoglobulin fold is a type of [1] structure.

Answer 4

[1] supersecondary

– hydrophobic interactions / beta sheets

Question 5

The [1] is a very common structural motif in a wide variety of proteins.

Answer 5

[1] Immunoglobulin fold

Question 6

ELISA is based on the principle of [1].

Answer 6

[1] Antibody binding to antigen

– secondary antibody will be antibody-enzyme complex to create color change

Question 7

The Chromatin Immunoprecipitation (ChIP) is used to investigate the interaction between [1] and [2].

Antibody bound to complex, isolate, probe microarray.

Answer 7

[1] protein

[2] DNA

Answer 8

[1] Polyclonal Antibodies

[2] Monoclonal Antibodies

Question 9

Multiple Myeloma
cancer of plasma cells
symptoms include elevated calcium, renal failure, anemia, and bone lesions (CRAB)
patients have a high concentration of [1] in their serum and secrete light chains called [2] in their urine

Answer 9

[1] monoclonal antibody (M protein)
[2] Bence Jones protein
– monoclonal light chains

Question 10

[1] is a genetic marker used in the production of monoclonal antibodies.
Fused cell MUST be positive for this marker (thus myeloma cells by themselves won’t grow)

Answer 10

[1] HGPRT

Question 11

Therapeutic mAbs Naming Convention:

• zumab = [1] Abs
• momab = [2] Abs
• ximab = [3] Abs
• mumab = [4] Abs

Answer 11

[1] Humanized
[2] Murine
[3] Chimeric
[4] Fully Human