Immunodiagnostics Flashcards
What are immunodiagnostics used for?
To assess patients with suspected immunological conditions
What are the clinical situations in which immune system components are assessed?
- Infection (seroconversion:antibodies produced against pathogens)
- Immunodeficiency: quantitative and qualitative measurements
- Allergy
- Autoimmune disease: autoantibodies and T cell autoreactivity
- Transplantation: HLA matching
What can be used for the detection of antibodies or antigens?
Agglutination and precipitation
What is the use of nephelometry?
The quantification of proteins in serum, plasma or cerebrospinal fluid
What is the use of immunoprecipitation.
Isolation of specific antigen from a mixture of antigens
What is the use of radial immunodiffusion?
Quantification of antigen
Nephelometry measures the formation of?
Immune complexes
Immunoprecipitation requires?
Immobilized antibody specific for the antigen of interest
In electrophoresis, antibodies migrate to the?
gamma region
Western blotting requires?
SDS and a polyacrylamide gel
Labeling proteins in western blotting requires?
Primary antibody specific for the antigen of interest
What does ELISA stand for?
Enzyme Linked Immunosorbent Assay
A typical enzyme used in ELISA assay is?
Horsearadish peroxidase
A sandwich immunoassay requires.
A labeled antibody and an antibody bound to a micotiter plate that can both bind to the same antigen at different regions
An example of a reporter tag used in the Fluorescent Microphere Assay is?
Fluorescent anti human IgG antibody
