Immuno Histology

Question 1

Granulocytes (3)

Answer 1

Neutrophils Eosinophils Basophils

Question 2

Agranulocytes (2)

Answer 2

Monocytes Lymphocytes

Question 3

Size ranking of blood elements

Answer 3

Monocytes > Eosinophils/Neutrophils > Lymphocytes/Basophils > Erythrocytes > Platelets

Question 4

Leukocyte % ranking

Answer 4

Neutrophils > Lymphocytes > Monocytes > Eosiniphils > Basophils

Question 5

Answer 5

Neutrophil

Question 6

Answer 6

Eosinophil

Question 7

Answer 7

Basophil

Question 8

Answer 8

Lymphocyte

Question 9

Answer 9

Monocyte

Question 10

Answer 10

Platelets

Question 11

Answer 11

Proerythroblast

Question 12

Answer 12

Basophilic erythroblast

Question 13

Answer 13

Polychomatophilic erythroblast

Question 14

Answer 14

Orthochomatophilic erythroblast

Question 15

Answer 15

Reticulocyte

Question 16

Answer 16

Promyelocyte

Question 17

Answer 17

Neutrophilic myelocyte

Question 18

Answer 18

Neutrophilic metamyelocyte

Question 19

Answer 19

Neutrophilic stab (band) cell

Question 20

Encapsulated lymphoid tissues (4)

Answer 20

1. Thymus
2. Tonsils
3. Lymph nodes
4. Spleen

Question 21

Diffuse Lymphoid Tissue

Answer 21

MALT (BALT, GALT)

Question 22

T-cell movement in Thymus

Answer 22

Cortex –> Medulla as they mature

Question 23

Blood Thymus Barrier

Answer 23

1. Capillary endothelium and basal lamina
2. Perivascular connective tissue (e.g., macrophages)
3. Thymic epithelial “reticular” cells and basal lamina

Question 24

Answer 24

Thymus (Capsule, Cortex, Medulla)

T-Cells, No B-cells

Question 25

Answer 25

Hassall’s corpuscle in Thymus: degraded epithelial cells in medulla

Question 26

Answer 26

Peyer’s Patch: Large lymphoid nodule (GALT) in submucosa of ileum

Almost all B-cells (Mostly making IgA into lumen - help build immune response)

Displaces connective tissue (blue) but is not encapsulated

Question 27

Answer 27

Lymphoid nodule (Mostly B-cells) in tonsil, surrounded by T-cells and APCs (macrophages)

Tonsils recognized by crypts; deep invaginations of the stratified squamous epithelium

Question 28

Answer 28

Lymph node medulla

A: Efferent lymphatic vessel

B: Medullary cord (lymphocytes and plasma cells)

C: Medullary sinus (macrophages and other cells)

Question 29

Answer 29

Lymph node cortex

A: Capsule

B: Afferent lymphatic vessel

C: Subcapsular sinus

D: Lymphoid nodules (B-cells)

Question 30

Answer 30

Lymph node paracortex (Thymus dependent region)

A: T cells

B: High-endothelial venule

Question 31

Answer 31

Spleen

A: Red pulp

B: Periarteriolar lymphoid sheath (PALS) (T cells)

C: Central arteriole

D: Lymphoid nodule of white pulp (B cells)

Question 32

Answer 32

Spleen:

GC = germinal center
MgZ = marginal zone
MnZ =mantle zone
PL = perilymphoid red pulp

Question 33

Thymus function

Answer 33

Site of maturation of T cells

Question 34

Lymph node function/structure

Answer 34

• Filters lymph
• Maintains and produces B cells and T cells

Question 35

High-endothelial venule

Answer 35

Specialized postcapillary venule in paracortex of lymph node with simple cuboidal epithelium

90% of lymphocytes leave bloodstream and enter lymph node here by diapedesis

Question 36

Spleen function

Answer 36

1. Filters blood
2. Stores and phagocytoses RBCs
3. Site of proliferation of B cells and T cells
4. Site of production of antibodies by plasma cells