Identification of Yeasts and Molds

Question 1

IoY: Morphological includes:

Answer 1

a. color of colonies
b. size and shape of cells
c. presence of capsule around the cell
d. production of hyphae, pseudohyphae, arthroconidia, and(or) chlamydospores

Question 2

Identification of yeast relies on a combination of

Answer 2

Morphological, Physiological and Biochemical characteristics

Question 3

IoY: Biochemical characteristics

Answer 3

a. assimilation and fermentation of sugars
b. assimilation of nitrate

Question 4

IoY: are based on sugar assimilation of isolates

Answer 4

Most commercial tests (eg., ID YST, bioMérieux)

Question 5

IoY: (MALDI-TOF)2

Answer 5

Matrix-assisted laser desorption ionization-time of flight mass spectrometry

Question 6

Most can be identified after growth in culture. Recognizable criteria for recognition often differ from the fundamental characteristics as the basic of classification.
1. Macroscopic characteristics viz. colonial form, surface color (obverse) pigmentation, reverse pigmentation, and growth rate.

Answer 6

Identification of Molds

Question 7

IoM: Factors that influence the colonial form

Answer 7

1. Culture medium
2. Incubation temperature
3. Age of culture
4. Amount of inoculum

Question 8

IoM: is important in speciation, hence it is important to select culture conditions which favor sporulation.

Answer 8

Sporulation

Question 9

IoM: promotes over production of mycelium, which results in loss of sporulation, in such cases, subculture to a low-nutrient medium (stimulate sporulation)

Answer 9

Sabouraud’s dextrose agar (SDA)