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Phase 2a > ICS - MIcrobiology 2 > Flashcards

ICS - MIcrobiology 2 Flashcards

1
Q

What does gram staining do?

A

Differentiate between gram positive and gram negative bacteria

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2
Q

Describe the gram staining process

A
  1. Fixation to microscope slide (heat/methanol)2. Primary stain - crystal violet3. Mordant - iodine4. Decolourisation - ethanol or acetone5. Counterstain - safranin
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3
Q

What colours does gram staining turn gram positive and negative bacteria?

A

Gram positive - purpleGram negative - pink

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4
Q

What bacteria does not stain with gram staining?

A

Acid fast bacilli, mainly mycobacteria (e.g. TB)

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5
Q

Why do mycobacteria not stain with gram staining?

A

Have a waxy, lipid cell wall that is impenetrable to the gram stain (also allows them to withstand phagolysosomal killing)

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6
Q

What stain is used for mycobacteria?

A

Ziehl-Neelsen stain

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7
Q

Describe the Ziehl-Neelsen stain process

A

Use heat to drive a dye (carbon fuchsin) inside the cell wall

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8
Q

What colours does Ziehl-Neelsen staining turn bacteria?

A

Acid fast bacteria (positive mycobacterium) - redNon-acid fast bacteria - blue

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9
Q

Define pathogen

A

Organism that causes or is capable of causing disease

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10
Q

Define commensal

A

Organism which colonises the host but causes no disease in normal circumstances

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11
Q

Define opportunist pathogen

A

Microbe that only causes disease if host defences are compromised

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12
Q

Define virulence/pathogenicity

A

How easily a pathogen is spread E.g. high virulence = easily spread e.g. measles

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13
Q

Define asymptomatic carriage

A

When a pathogen is carried harmlessly at a tissue site where it causes no disease

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14
Q

Describe cocci

A

Round-shaped bacteria. Can be individual, as pairs, (diplococci), in chains or clusters

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15
Q

Describe bacilli

A

Rod-shaped bacteria. Can be individual, chains, vibrio (curved), spirochaete (spiral)

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16
Q

Describe blood agar

A
  • Contains sheep/horse blood- Provides a good medium for growing many different types of bacteria (non selective)
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17
Q

Describe chocolate agar

A
  • Blood agar heated to 80 degrees celsius for 5 minutes- Releases nutrients into agar and makes it easier to grow certain organisms (fastidious bacteria)
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18
Q

What are 2 examples of bacterias that grow better on chocolate agar?

A
  1. Haemophilus influenza2. Neisseria meningitidis
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19
Q

Define fastidious bacteria

A

Bacteria that is hard to grow because they have complex/restricted nutritional and/or environmental requirements

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20
Q

Describe MacConkey agar

A
  • Contains bile salts (inhibits gram positive), lactose and pH indicator- Designed primarily to grow gram negative bacilli- Enables differentiation of gram negative bacilli into lactose fermenters and non-lactose fermenters
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21
Q

What colour are gram negative bacilli on MacConkey agar?

A

Lactose fermenting - redNon-lactose fermenting - white/colourless

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22
Q

Describe CLED agar

A
  • Cysteine lactose electrolyte deficient- Use to differentiate microorganisms in urine - Enables differentiation of lactose fermenting and non lactose fermenting gram negative bacilli
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23
Q

What colour are gram negative bacilli on CLED agar?

A

Lactose fermenting - yellowNon lactose fermenting - blue

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24
Q

Describe Sabouraud’s agar

A
  • For fungi- Selective media
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25
Q

Describe XLD agar

A
  • Xylose lysine deoxycholate agar- Selective media- Contains indicator
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26
Q

Describe Lowenstein-Jensen agar

A
  • Specifically used for culture of mycobacterium species- Selective media
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27
Q

Which sites are sterile in the body?

A
  • Blood- CSF- Joints- Lower resp tract- Pleural fluid- Peroneal cavity- Urinary tract
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28
Q

Which sites are colonised with flora in the body?

A
  • GI tract- Skin- Oral cavity (upper resp tract)- Vagina- Urethra- Bladder
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29
Q

What is alpha beta gamma haemolysis?

A

Used to indicate different types of streptococci on blood agar plates

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30
Q

What test is used to differentiate beta haemolytic streptococci?

A

Streptoccoci Lancefield grouping test

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31
Q

What are the results of streptococcus lancefield grouping test?

A

Group A = strep. pyogenesGroup B = strep. agalactiaeGroup C = strep. dysgalactiaeGroup D = enterococcus

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32
Q

Describe the results of alpha beta gamma haemolysis

A
  • Alpha - green/brown- Beta - clear- Gamma - neither (may be slightly discolouration in the medium)
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33
Q

What is the optochin test?

A
  • Used in the identification of streptococcus pneumoniae- Filter paper discs are impregnated with ethylhydrocupreine hydrochloride and applied directly to inoculated plates before incubation
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34
Q

Describe the results of the optochin test

A
  • If a culture is resistant to optochin, the disc won’t prevent cultures growing- If a culture is sensitive (susceptible) to optochin, the disc prevents cultures growing (streptococcus pneumoniae)
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35
Q

What is the coagulase test?

A

Used to differentiate bacteria that produce coagulase (e.g. staphylococcus aureus) from those that do not (e.g staphylococcus epidermidis/saprophyticus)

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36
Q

What is coagulase?

A

Enzyme-like protein that causes plasma to clot by converting fibrinogen to fibrin

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37
Q

Describe the results of the coagulase test

A

If coagulase is present, clotting will occur

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38
Q

What is the catalase test?

A

Used to differentiate bacteria that produce catalase (e.g. staphylococci) from those that do not (e.g streptococci)

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39
Q

What is catalase?

A

Enzyme that catalyses the release of oxygen from hydrogen peroxide

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40
Q

Describe the results of the catalase test

A

Hydrogen peroxide (H2O2) is added and if catalase is present, H2O2 will be broken down into 2H2O + O2 (gas bubbles)

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41
Q

Describe the characteristic features of gram positive bacteria

A

Single membrane and thick peptidoglycan layer

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42
Q

Describe the characteristic features of gram negative bacteria

A

Double membrane, thin peptidoglycan layer and lipopolysaccharides (endotoxin area)

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43
Q

What does the endotoxin area of gram negative bacteria comprise?

A

Terminal sugars. O antigen and Lipid A

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44
Q

Between what temperatures and pH range can bacteria grow?

A

-80 to +80 degrees celsius and pH of 4-9

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45
Q

What are the 3 phases of bacterial growth?

A
  1. Lag phase2. Exponential phase3. Stationary phase
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46
Q

Give an example of slow growing bacteria

A

TB

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47
Q

Give 2 examples of fast growing bacteria

A

E.coli and Staphylococcus. aureus

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48
Q

Give 2 functions of pili

A
  1. Help adhere to cell surfaces2. Plasmid exchange
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49
Q

What is the primary function of flagelli?

A

Movement

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50
Q

What is the primary function of the polysaccharide capsule?

A

Protection - prevents MAC or opsonisation molecules attacking

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51
Q

What types of bacteria release endotoxin?

A

Gram negative bacteria

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52
Q

What types of bacteria release exotoxin?

A

Gram positive and gram negative bacteria

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53
Q

What are endotoxins?

A

Component of bacteria’s outer membrane released when bacteria are damaged. Less specific and toxic to the host. Heat stable

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54
Q

What are exotoxins?

A

Proteins secreted that are specific and heat labile

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55
Q

What are plasmids?

A

Circular pieces of DNA that often carry genes for antibiotic resistance

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56
Q

Gram positive cocci can be divided into…?

A

Staphylococcus (clusters) and streptococcus (chains)

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57
Q

How do you distinguish between staphylococcus and streptococcus

A

Catalase test:Staph = catalase +ve (gas bubbles)Strep = catalase -ve (no change)

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58
Q

How do you further distinguish between staphylococci bacteria?

A

Coagulase test:+ve = Staphylococcus aureus-ve = all others (e.g. staph. epidermidis, staph. saprophyticus )

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59
Q

How do you further distinguish between streptococci bacteria?

A

Blood agar haemolysis:Alpha = e.g. streptococcus pneumoniaeBeta = e.g. streptococcus pyogenes/agalactiaeGamma = streptococcus bovis

60
Q

How do you further distinguish between alpha haemolytic streptococci?

A

Optochin test:Resistant = viridans strepSensitive = streptococcus pneumoniae

61
Q

Give 4 examples of gram positive bacilli

A
  1. Bacillus cereus (B. cereus)2. Listeria3. Diphtheria4. Clostridium (C. diff)
62
Q

Give 3 examples of gram negative cocci

A
  1. Neisseria gonorrhoeae (diplococci)2. Neisseria meningitidis (diplococci)3. Moraxella catarrhalis
63
Q

How do you distinguish between gram negative bacilli?

A

MacConkey agar:Pink (+ve) = lactose fermenting e.g. E.coli, klebsiella pneumoniaeWhite (-ve)= non lactose fermenting e.g. shigella, salmonella

64
Q

How do you further distinguish between non lactose fermenting bacteria?

A

Oxidase test:+ve = pseudomonas aeruginosa-ve = shigella, salmonella, proteus

65
Q

How do you differentiate between negative oxidase non-lactose fermenting bacteria?

A
  • Cultivate on XLD agar- Red with black centres = salmonella- Only red = shigella
66
Q

What is the oxidase test?

A

Test used to determine if a bacterium produces certain cytochrome c oxidases

67
Q

What does it mean if gram negative bacilli have a H antigen?

A

They are motile and have a flagellum

68
Q

What is a virus?

A

An infectious, obligate intracellular parasite comprising genetic material surrounded by a protein coat and/or membrane

69
Q

When not inside an infected cell, viruses exist as … which consist of …

A

Virions which consists of genetic material (DNA or RNA) and a protein coat (capsid)

70
Q

What shapes can viruses be?

A

Helical, icosahedral and complex

71
Q

What is an enveloped virus?

A

Virus surrounded by a lipid coat derived from the plasma membrane of the host cell

72
Q

Give 2 examples of enveloped viruses

A
  1. Influenza2. HIV
73
Q

Give 2 examples of non enveloped viruses

A
  1. Adenovirus2. Parvovirus
74
Q

What is a nucleocapsid?

A

The protein coat of a virus (capsid) and the nucleic acid of the virus

75
Q

Are viruses living?

A

NO - they do not feed or respire and cannot reproduce independently

76
Q

What are the 5 steps of virus replication?

A
  1. Attachment to specific receptor2. Cell entry3. Host cell interaction and replication4. Assembly of virion5. Release of new virus particles
77
Q

Name 5 ways viruses can cause disease

A
  1. Direct destruction of host cells2. Modification of host cells3. ‘Over reactivity’ of immune system4. Damage through cell proliferation5. Evasion of host defences
78
Q

Give an example of direct destruction of host cells

A

Poliovirus causes host cell lysis and death after a viral replication period of 4 hours

79
Q

Give an example of modification of host cells

A

Rotavirus causes villi to atrophy and epithelial cells to flatten. This decreases the small intestine surface area and prevents nutrients (e.g. sugar) from being absorbed to cause a hyperosmotic state and profuse diarrhoea

80
Q

Give an example of ‘over reactivity’ of immune system

A

Hepatitis B causes jaundice, pale stool, dark urine, RUQ pain, fever/malaise and itching

81
Q

Give an example of damage through cell proliferation

A

Human papillomavirus (HPV) is associated with cervical, penile, anal, vaginal, vulval and head/neck cancer - the insertion and replication of its DNA leads to dysplasia and neoplasia which leads to cell proliferation and local/metastatic spread

82
Q

Give 6 examples of evasion of host defences

A
  1. Latency2. Cell to cell spread3. Antigenic variability (ability to change surface antigens)4. Prevention of host cell apoptosis5. Down regulation of interferon and other intracellular host defence proteins6. Interference with host cell antigen processing pathways
83
Q

What are protozoa?

A

Microscopic unicellular eukaryotes

84
Q

What are the 5 major groups of protozoa?

A
  1. Flagellates2. Amoebae3. Sporozoa4. Cilliates5. Microsporidia
85
Q

What is malaria transmitted by?

A

Female anopheles mosquitos

86
Q

What are the 5 species of malaria?

A
  1. Plasmodium falciparum2. Plasmodium ovale3. Plasmodium vivax4. Plasmodium malariae5. Plasmodium knowlesi
87
Q

How is malaria diagnosed?

A

Look for trophozoites on blood film under a light microscope

88
Q

Describe the use of the thick film in diagnosing malaria

A

Works out if the patient has malaria or not

89
Q

Describe the use of the thin film in diagnosing malaria

A

Works out what species of malaria the patient has

90
Q

What are 4 signs of malaria?

A
  1. Anaemia2. Jaundice3. Hepatosplenomegaly4. Black water fever
91
Q

What are 8 symptoms of malaria?

A
  1. FEVER2. Chills3. Headache4. Myalgia5. Fatigue6. Diarrhoea7. Vomiting8. Abdominal pain
92
Q

Describe the lifecycle of malaria

A
  • Mosquito bites infected person and ingests plasmodium gametocytes- These develop into sporozoites- Mosquito bites another person and injects the sporozoites- The sporozoites infect hepatocytes- Infected hepatocyte becomes a schizont- The schizont bursts and infects RBCs (where plasmodium becomes trophozoites and rupture RBCs and reinfect others)- Some trophozoites develop into gametocytes which are taken up by other mosquitos
93
Q

Which is the most severe species of malaria?

A

Plasmodium falciparum

94
Q

How are RBCs infected with plasmodium falciparum different?

A

Have proteinaceous knobs on the surface that bind to endothelial cells and other RBCs. This can cause small vessels to become obstructed causing hypoxia and microinfarcts

95
Q

Name 4 ways you can treat malaria

A
  1. IV artesunate2. IV quinine and doxycycline3. Riamet4. PO chloroquine
96
Q

Which species of malaria can lie dormant?

A

Plasmodium ovale and plasmodium vivax can form hypnozoites in the liver

97
Q

What makes up the cell wall of fungi?

A

Chitin and glucans (polysaccharides)

98
Q

How do fungi move?

A

By growing across or through structures OR by dispersion in air/water

99
Q

What forms do fungi exist in?

A
  1. Yeast - single cell that divides via budding2. Moulds - form multicellular hyphae or spores
100
Q

What are worms also known as?

A

Helminths

101
Q

What are the 3 groups of worms?

A
  1. Nematodes (round worms)2. Trematodes (flat worms a.k.a flukes)3. Ceratodes (tapeworms)
102
Q

What are nematodes and what are the types?

A

Round worms- Intestinal variety (e.g. threadworm)- Larva migrans - Filarial tissue worms (sometimes considered to be a 4th group)

103
Q

What are trematodes and what are the types?

A

Flat worms (flukes)- Blood (cause schistosomiasis)- Liver- Lung- Intestinal

104
Q

What are ceratodes and what are the types?

A

Tapeworms - Non invasive (e.g. sit in the bowel) - Invasive (invade the body)

105
Q

Can adult worms replicate inside the body?

A

Not without a period of development outside the body

106
Q

What is the pre-patent period of worms?

A

Interval between infection and appearance of eggs/larvae in stool

107
Q

What do glycopeptides (antibiotics) do?

A

Inhibit cell wall synthesis

108
Q

How are intestinal nematodes transmitted?

A

From human to human via eggs or larvae (not usually infectious when first passed and has to undergo a period of development in the soil). Faecal-oral spread

109
Q

How are worms usually diagnosed?

A
  1. Stool microscopy for eggs2. Seeing the worm itself (if big enough e.g. intestinal nematodes)
110
Q

What do intestinal nematodes cause and how are they treated?

A

Malnutrition (travel around the body)Treatment: Mebendazole, piperazine, pyrantel, levasimole

111
Q

What is ground itch?

A

Papules and itching at the site of entry on the larvae (e.g. feet) caused by hookworm (intestinal nematode that travels around the body). The commonest cause of iron deficiency anaemia in the world

112
Q

How is hookworm treated?

A

Mebendazole, pyrantel

113
Q

What is enterobius vermicularis also known as?

A

Pinworm or threadworm (intestinal nematodes that don’t travel, goes from mouth straight through to anus but can poke into appendix or vaginal/uterus penetration)

114
Q

What is hyperinfection syndrome?

A

Associated with auto-infection and an immunocompromised state e.g. due to advanced HIv or corticosteroid therapy

115
Q

What are 3 symptoms of hyperinfection syndrome?

A
  1. Diarrhoea2. Weight loss3. Malabsorption
116
Q

Give 2 examples of glycopeptides (antibiotics)

A
  1. Vancomycin2. Teicoplanin
117
Q

What do beta lactams (antibiotics) do?

A

Inhibit cell wall synthesis by disrupting peptidoglycan synthesis

118
Q

What are the 4 types of beta lactams (antibiotics)?

A
  1. Penicillins (e.g. amoxicillin, flucloxacillin, benzylpenicillin)2. Cephalosporins (e.g. cephalexin, cefotaxime)3. Carbapenems (e.g. estapenem, imipenem)4. Monobactams (e.g. aztreonam)
119
Q

What do antibiotics e.g. trimethoprim and sulphonamides do?

A

Inhibit nucleic acid synthesis by inhibiting folate synthesis

120
Q

What do antibiotics e.g. ciprofloxacin and fluroquinolones do?

A

Inhibit nucleic acid synthesis by inhibiting DNA gyrase

121
Q

What do antibiotics e.g. rifamipcin do?

A

Inhibit nucleic acid synthesis by binding to RNA polymerase

122
Q

What do antibiotics e.g. metronidazole do?

A

Inhibit nucleic acid synthesis by causing breaks in DNA strands

123
Q

Which 4 types of antibiotics inhibit protein synthesis?

A
  1. Chloramphenicol2. Macrolides (e.g. clarithromycin, erythromycin)3. Tetracyclines (e.g. doxycycline)4. Aminoglycosides (e.g. gentamicin, streptomycin)
124
Q

What do bactericidal antibiotics do?

A

Kill bacteria e.g. antibiotics that inhibit cell wall synthesis

125
Q

What is a consequence of bactericidal antibiotics?

A

Can lead to the release of endotoxin (bits of cell wall) which cause an increase in antigenic load –> aggressive/dangerous inflammatory response

126
Q

What do bacteriostatic antibiotics do?

A

Prevent growth of bacteria e.g. antibiotics that inhibit protein synthesis/DNA replication/metabolism

127
Q

Why might bacteriostatic antibiotics be better?

A

Reduced toxin production and endotoxin surge less likely

128
Q

Define minimum inhibitory concentration

A

Lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation

129
Q

Give 4 examples of antibiotic resistance

A
  1. Change antibiotic target2. Destroy antibiotic3. Prevent antibiotic access4. Remove antibiotic from bacteria
130
Q

Describe and give an example of changing antibiotic targets

A
  • Bacteria change the molecular configuration of antibiotic binding sites or masks them- E.g. flucloxacillin can no longer bind to PBP of staphylococci
131
Q

Describe and give an example of destroying antibiotics

A
  • Antibiotic destroyed or inactivated- E.g. penicillins and cephalosporins have beta lactam rings which become hydrolysed by bacterial enzymes (beta lactamase) so that they cannot bind to PBP
132
Q

What does PBP stand for?

A

Penicillin binding proteins (in bacteria)

133
Q

Describe and give an example of preventing antibiotic access

A
  • Bacterial membrane porin channel is modified e.g. in size, number, selectivity- E.g. gram negative bacteria against glycosides
134
Q

Describe and give an example of removing antibiotics from bacteria

A
  • Proteins in bacterial membranes act as exports/efflux pumps to reduce the levels of antibiotics- E.g. enterobacteriacae resistance to tetracyclines
135
Q

Describe intrinsic resistance by bacteria

A
  • Natural resistance- All subpopulations of a species are equally resistant
136
Q

Give an example of intrinsic resistance by bacteria

A

Gram negative bacteria have an outer membrane that cannot be penetrated by vancomycin

137
Q

Describe acquired resistance by bacteria

A
  • Bacteria which were previously susceptible obtain the ability to resist the activity of a particular antibiotic- Only certain subpopulations of a species will be resistant
138
Q

Give an example of acquired resistance by bacteria

A
  • Spontaneous gene mutation- Horizontal gene transfer (conjugation, transduction, transformation)
139
Q

When diagnosing viral and bacterial infections, which swabs are best?

A

Viral - green swabsBacterial - charcoal swabs

140
Q

How often do bacteria divide?

A

Every 30-60 minutes

141
Q

What does the cell wall of mycobacteria contain?

A

Lipoarabinomannan

142
Q

What may mycobacteria cause?

A

Meningitis

143
Q

Antimicrobial resistance is spread by…

A

Plasmid mediated gene transfer

144
Q

What does MRSA refer to and how is it usually treated?

A

Methicillin (flucloxacillin) resistant staphylococcus aureus - treated with glycopeptides (e.g. vancomycin)

145
Q

What is the first line treatment for streptococcus pyogenes?

A

Penicillin

146
Q

EBV infection can cause…

A

Infectious mononucleosis (mono)

147
Q

What would tests of infectious mononucleosis show?

A
  • Atypical lymphocytes on a blood film- Detectable EBV IgM in serum

Phase 2a (29 decks)

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