HW: virus replication & diseases

Question 1

Which is NOT true regarding plaque assays?

Answer 1

plaque assays are used to quantify lysogenic phages as well as lytic phages

Question 1

What is an effective way of growing an animal virus?

Answer 1

in a primary cell culture

Question 2

true about plaque assays

Answer 2

viral titers are PFU
plaques are clear zones seen bc viruses lysed the host cells in the area
top agae containing a dilution of virions and bacteria is poured on the agar plate

Question 3

animal viruses typically enter the cell by

Answer 3

membrane fusion or endocytosis

Question 4

there are no HIV vaccines

Answer 4

true

Question 5

extracellular state of a virus

Answer 5

virion

Question 6

enzyme used by retroviruses to transfer info from RNA to DNA

Answer 6

reverse transcriptase

Question 7

Which of the following is the LEAST common reservoir for the rabies virus in the United States?

Answer 7

chicken

Question 8

The rhabdovirus that causes rabies is a

Answer 8

bullet shaped virus

Question 9

two types of symmetry in viruses
rod shaped: _____
spherical:_______

Answer 9

helical/ icosahedral

Question 10

Reassortment of a virus that results in antigenic shift most commonly occurs in

Answer 10

any animal infected with more than one virus strain

Question 11

Hantavirus is transmitted to humans by

Answer 11

inhalation of rodent excreta

Question 12

a viral capsid is composed of individual units of _____ called_____

Answer 12

proteins//capsomeres

Question 13

difference between bacteriophage and animal viruses

Answer 13

• many animal viruses replicate their genome within their host cell nucleus
• the entire virion of animals viruses enters the host cell
• many animal viruses are enveloped

Question 14

HIV-1

Answer 14

is a retrovirus, targets helper T cells and macrophages

Question 15

concentration of viruses

Answer 15

titer