Hunting the gene extra facts

Question 1

CRISPR describe

Answer 1

Part of bacterial adaptive immune system against bacteriophage infection, used together with endonuclease cas9 that getsrecruited to specific sequences by guide RNA CRISPR.
CRISPR Cas9 important in eukaryotic cells for patients with well-defined mutation in well-defined disease eg. sickle cell disease

Question 2

Mendel citations

Answer 2

Only 3 times in first 35 years published in obscure journal :(

Question 3

Mitotic recombination describe

Answer 3

To look at this do clonal analysis of populations of cells in the same tissue with different genotypes
Cancer cells are genotypically distinct

Question 4

Three parent baby

Answer 4

I know this well, put in essay about mendelian inheritance

Question 5

Why is there much less human mitochondrial DNA than yeast mitochondrial DNA?

Answer 5

Mitochondria very metabolically active with mutagenic compounds so we want to remove DNA to the nucleus if possible where there are more DNA repair mechanisms

Question 6

Horizontal gene transfer and biofilm

Answer 6

New mutations spread quickly in biofilm due to horizontal gene transfer
eg. bacteria that cause pneumonia once they reach certain population size they create protective layer of peptidiglycan, protecting neighbours and protecting themselves as antibiotics can’t reach.