BoC Enzymes Flashcards
What are 6 main types?
oxoreductases, transferases, hydrolases, lyases, iosomerase, ligase
Draw out enzyme reaction scheme
see notes
What is kd
k2/k1
What is km?
(k2+k3)/k1
k3 also known as
kcat
What 2 monosaccharides do bacterial cell walls contain?
N-acetylglucosamine and N-acetyl muramic acid
Draw bacterial cell wall
see notes
Lysozyme binds…
the sugar moiety of the peptidoglycan layer, has a hydrophobic active site cleft
Draw lysozyme action basic
see notes
This mechanism illustrates principles of…
Geometric effects, nucleophilic attack, acid catalysis
What is an example of an irreversible inhibitor?
Nerve agent sarin, interacts with acetylcholinesterase
What is a suicide inhibitor and what is an example of one?
Enzyme starts to catalyse reaction of inhibitor, forms covalent bond, can’t proceed further, stuck with inhibitor eg. penicillin for glycopeptide transferase
What is an eg of a competitive inhibitor?
Methotrexate, a chemo drug that mimics folic acid, competes with folic acid for thymine synthesis, folic acid essential for DNA synthesis
How can enzymes be controlled
(de)phosphorylation, negative feedback (often genetic control with TF), Activation of zymogens (inactive enzymes that can be switched on but not off unless destroyed), allosteric binding, compartmentalisation
Describe how haemoglobin is cooperative (draw diagram of the concerted and sequential model)
see notes