cell cycle checkpoints

Question 1

1st step Prokaryotic cell division

Answer 1

uncoil DNA, replicate from OriC

Question 2

2nd step

Answer 2

DNA copies move to opposite poles and attach to cell wall

Question 3

3rd step

Answer 3

Plasmid DNA replicated and polarised

Question 4

4th step

Answer 4

FtsZ ring grows

Question 5

5th step

Answer 5

FtsZ closes, new cell wall deposited, calls separate

Question 6

Themes in cell cycle

Answer 6

Phosphorylation, ubiquitination, positive feedback

Question 7

Responds to ds breaks in DNA, phosphorylates Chek1/2

Answer 7

ATM kinase

Question 8

Responds to ss DNA, phosphorylates CHEK1/2

Answer 8

ATR kinase

Question 9

CHEK 1 and 2… (at G1/S)

Answer 9

phosphorylate p53

Question 10

p53… (at G1/S)

Answer 10

promotes p21 expression

Question 11

p21… (at G1/S)

Answer 11

inhibits CDK2 and CDK4/6, G1/S transition prevented, more time for repair

Question 12

CHEK 1 and 2… (at G2/M)

Answer 12

phosphorylate p53, phosphorylate CDC25

Question 13

p21… (at G2/M)

Answer 13

Inhibits CDK2

Question 14

p53… (at G2/M)

Answer 14

promotes p21 expression, interacts with 14.3.3 proteins

Question 15

14.3.3…

Answer 15

hides CDC25 in cytoplasm

Question 16

CDC25 normal function

Answer 16

activates cyclinB/CDK1