HPV pathogenesis Flashcards
What type of host cancer related protein do viral oncoproteins target
Tumour suppressor
Maintence of proliferative ability: What is the major function of E6 and E7
maintain undifferentiated and differentiated cells in the suprabasal region of the skin in the cell cycle, they do this via interacting with many cellular targets.
Does E7 have intrinsic enzymatic or DNA binding properties
No
What cell factors does E7 bind to
Best characterised are members of the retinoblastoma family of tumour suppressor proteins (pRb, p107, p130).
E7 binds to retinoblastoma family of tumour suppressor proteins through what motif of amino acids
LCCXE motif
What do the retinoblastoma tumour suppressor protein family control
G1-S phase transition by regulating activity of E2F family transcription factors.
A major consequence of efficient targetting of pRb by E7 is an increase in levels of what tumour suppressor protein
p53
Best studies E6 functions require interactions with host what
E3 ubiquitin ligases
E6 mediated degradation of what
p53
What does E6 require to degrade p53
Ub ligase E6-AP
Some E6 proteins contain what motif at their extreme C-terminus
PDZ-binding motif
Mutations of PDZ binding motif within E6 leads to
- Reduced growth rate
- Loss of viral episomes
- Frequent integration of viral genome into host chromosome
E6 activates the S______ transcription factor
STAT3
HPV targets the H_____ pathway
Hippo (YAP1)
E5 forms a novel ion channel otherwise called a
Viroporin
E5 maintains what receptor expression and singalling
EGFR (epidermal grwoth factor receptor) –> helps cells grow and divide
Pre-cancerous lesions are called what abbrevation
CIN- cervical intraepithelial neoplasia
