HCV molecular biology Flashcards
What HCV genotype was cloned from patient with fulminant hepatitis in 2001?
Genotype HCV 2a
What is JFH-1
Japenese fulminant hepatitis (Genotype 2a HCV)
How well does SGRs from JFH-1 replicate?
Very well without culture adaptation
In what cells do JFH-1 replicons replicate
Hela/293 cells
Does full length JFH-1 RNA transfection produce ifnectious particles?
Yes
What are cytoplasmic host proteins involved in HCV entry?
GAG
LDL-R
SRB1
Claudin
What host protein which is found in tight junctions between cells is involved in HCV entry?
Occludin
Function of HCV: Core
Nucleocapsid protein, nucleic acid binding, associated with ER membrane/lipid droplets
Function of HCV: E1/2
Envelope glycoproteins, receptor binding
Function of HCV: p7
Ion channel
Function of HCV: NS2
Multiple TM helicases, cleaves itself from NS2/3 precursor. Assebly and release
Function of HCV: NS3/4A
Helicase/protease complex, it is a stable complex. Ns3 is a protease that cleaves NS3-NS4A-NS4B-NS5A-NS5B junctions
Function of HCV: NS4B
Multiple membrane spanning protein, membrane rearrangement, and membranous “web”
Function of HCV: NS5A
Key component of RNA replication complex, binds viral RNA, viral assembly
Function of HCV: NS3B
RdRp, catalyses production of +ve and -veRNA strands
What struvture is at 5’ UTR of HCV genome?
IRES
What is IRES for?
Cap independent initiation of translation
What is at the 3’ UTR of HCV genome? Function?
Poly U/UC which has a role in negative strand replication
What does Poly U/UC bind on HCV?
NS5A and NS5B
What is the membranous web?
Double membraned vesicles, concentrated relpication component
How many NS5B are to 1 -veRNA strand in membranous web?
1000 NS5B : 1 RNA
What pathway potentially is involved in virus budding into intracellular vesicles?
ESCRT
