HPCT QUIZ 2ND Flashcards
This section may be cut from tissues that have been
fixed and frozen with CO2 or for fresh tissues frozen
with a cryostat.
A. Celloidin section
B. Frozen section
C. Paraffin section
D. Gelatin section
E. NOTA
Frozen section
Sections are not left on the water bath for a long time
due to:
A. To avoid undue expansion of the tissue
B. To avoid distortion of tissue
C. Both
D. NOTA
To avoid undue expansion of the tissue and to avoid distortion of the tissue
BOTH
Select which among the ff is a common component
of an aqueous media together with its correct use
A. Glycerol-prevent cracking
B. Glycerin-prevent drying
C. Gum Arabic- increase Refractive Index
D. Sugar-solidify medium
Glycerol
What is the specific pH used for Citric-Acid-Citrate
Buffer solution?
A. 4.5 - to neutralize and protect specimen from the
acidic component of solution
It is a semi-translucent, ground glass or hyaline
substance made up of chondroitin
A. Fibrin
B. Fibrinoid
C. Hyalin
D. Amyloid
D. Amyloid
Open muscle biopsies are usually received from the
operating room in a surgical clamp for orientation and
divided in three parts. What specific segment is fixed in
glutaraldehyde and is submitted for electron
microscopy?
A. Middle segment
B. Small segment
C. Large segment
D. Remaining segment
E. NOTA
B. Small segment
This type of glial cell represents the major
supporting cells in the brain.
A. Astrocytes
B. Microglia - major phagocytic cell
C. Myelin - insulating layer that modifies nerve
conduction
D. Ependymal cell - located in the line of ventricles of
the brain
A. Astrocytes
Clearing is done by:
A. 10 dips in alcohol
B. Gradual run through decreasing concentration of
alcohol
C. 2 changes of xylol, 2 hours each
D. 2 or 3 changes of xylene for 1 hour
D. 2 or 3 changes of xylene for 1 hour
Choose the answer with the correct association
between the difficulties encountered and its cause or the
fault that is made
A. Frozen tissue chips into fragments when cut;
freezing is not adequate
B. Frozen tissue crumbles and comes off the block
holder when cut; tissue is frozen too much
C. Tissue shrinks away from wax when trimmed;
insufficient infiltration
D. Tissue is opaque and there is presence of alcohol;
insufficient clearing
D. Tissue is opaque and there is presence of alcohol;
insufficient clearing
Which of the ff will affect the speed of penetration of
the clearing agent?
A. Temperature
B. Viscosity
C. PH
D. AOTA
B. Viscosity
This clearing agent can cause aplastic anemia
a. Benzene
b. Xylol
c. Toluene
d. Chloroform
a. Benzene
Dirty knife edge can cause this fault
Sections roll up
Sections adhere to knife or other parts of the microtome can be caused by? Except:
Knife edge is dirty
Knife edge is dull
Knife is too high
Knife tilt is too low
Knife tilt is too low
Celloidin section does cut come off in ribbons
False
Do not come off in ribbons dapat
Ultrathin microtome is widely known for cutting sections for electron microtome. What is the specific type of knives used in this type of microtome
Diamond knives
Optimum temperature of the water bath that is used to float tissue cut from the microtome
45-50C
For proper cutting, specific clearance angle must be used. Which of the ff angle is considered as the most effective
0-15 degrees
Process in histotechnology involves trimming and cutting embedded tissues into uniformly thin slices or sections
Microtomy
Kind of microtome that is ideal to use for unembedded frozen section
Freezing microtome
Microtome for paraffin embedded tissue
Rocking microtome for large and rotary for small
Microtome for celloidin sections
Sliding microtome
There are certain safety measures in handling the microtome to avoid accidents. What safety measure could be done to avoid getting injured when changing blocks?
Use hand wheel lock
What kind of microtome knife is used for cutting
resin blocks for electron microscopy?
Diamond knives
Which of the ff are important measures that are needed to be performed after embedding the tissue blocks and solidifying the paraffin wax, except:
Take out both the thin and thick sections
Remove the wax Block from the mold
Note the identification number
Cut off the excess wax
Take out both the thin and thick sections
Becomes milky when incomplete dehydration
Xylene
What factor should be met when doing the floatation of section
Water should be free from bubbles
Celloidin embedding is another technique besides paraffin embedding. Compared with paraffin embedding, what could be its advantage in tissue processing?
Completely avoids the use of heat
Possible reason why there is an uneven or unequal
thickness of the sections produced
a. Clamp set screw on knife or block holder is loose
b. Blocks are too large
c. Tilt of knife is too great
d. Blocks are too hard
e. AOTA
f. NOTA
Tilt of knife is too great
Part of microtome where tissue is held in position
Block holder
Most dangerous type of microtome due to the movable exposed knife
Standard-sliding microtome
Biconcave and plane-concave knifes are both used for paraffin sections. THe less concave side of plane-concave knife is used for celloidin embedded tissue blocks
Both statement are correct
Plane concave - celloidin
Biconcave - paraffin
-Glass and diamond knives - EM
The static electricity is due to low atmospheric
humidity because of
Sections adhere to the knife
Knife edge is dull
Knife tilt is too great
knife edge is dirty
AOTA
A type of block-out mold that is made up of interlocking plates resting on flat metal base
Compound embedding unit
Two L-Shaped brass arranged on a flat metal - Leuckhart’s
Special stainless steel base mold fitted with plastic - Plastic embedded rings and base mold