How Do We Find an Unknown Concentration?

Question 1

Conduct __________ for a solution of known concentration, and determine the absorbance of each sample using a ___________.

Answer 1

a series of dilutions, spectrophotometer.

Question 2

Since ___________ is directly proportional to __________ (______ law), you will be able to plot your data on the graph linearly. This will be your _____________

Answer 2

absorbance, concentration, Beer’s, Standard Curve

Question 3

Using this standard curve as a trend line, you will be able to estimate the unknown ___________ associated with a __________

Answer 3

x value (concentration), known y value (absorbance)

Question 4

Now that you have everything set up, you use the ________ to find the ________ of the _________.

Answer 4

spectrophotometer, absorbance, unknown concentration sample

Question 5

Then use the newly found absorbance to estimate the concentration using the ________

Answer 5

Standard Curve